The MS Grade Trypsin Protease from MtoZ Biolabs serves as the gold-standard protease for amino acid sequence analysis and protein identification in LC-MS/MS-based proteomics. It delivers highly efficient and specific digestion while ensuring exceptionally clean mass spectrometry data quality.
Product Overview
Trypsin is one of the most essential processing enzymes in modern proteomics workflows, responsible for cleaving proteins into peptides suitable for LC-MS/MS detection. However, non-MS-grade or standard-grade trypsin preparations often introduce a series of issues that compromise data integrity, including:
1. Background Contamination and Autolysis Interference
Non-MS-grade preparations may contain contaminating proteases or autolysis products from trypsin itself. These non-target peptides generate high-intensity background signals in MS spectra, interfering with peptide identification and quantitative analysis.
2. Non-Specific Cleavage
Lower-purity trypsin may cleave at unintended residues (e.g., after aspartic acid or glutamic acid), resulting in unpredictable peptide products that increase search complexity and reduce identification confidence.
3. Batch-to-Batch Inconsistency
Ambiguous enzymatic activity definitions and production variability can lead to inconsistent digestion efficiency across batches, affecting reproducibility and cross-study comparability.
4. Mass Spectrometry-Interfering Additives
Stabilizers such as glycerol, salts, or other excipients may suppress ionization efficiency during MS analysis, reducing sensitivity and dynamic range.
Advantages
✅ Ultra-High Purity with Minimal Background
The MS Grade Trypsin Protease from MtoZ Biolabs undergoes multiple chromatographic purification steps to minimize contaminating proteases and autolysis products. In LC-MS/MS analysis, peptides originating from the enzyme itself are nearly undetectable, ensuring high signal-to-noise ratios and exceptionally clean spectra.
✅ Outstanding Cleavage Specificity
Trypsin from MtoZ Biolabs cleaves strictly at the C-terminus of lysine (K) and arginine (R) residues (except when followed by proline), providing near-100% specificity. This ensures predictable peptide generation and substantially simplifies downstream database searching and bioinformatic interpretation.
✅ Reliable Performance Through Rigorous QC
Each batch is validated through BAEE activity assays and mass spectrometry compatibility testing, guaranteeing exceptional batch-to-batch consistency. Long-term projects can therefore maintain reproducible digestion efficiency and identification depth.
✅ High Enzymatic Efficiency Across Diverse Conditions
The enzyme retains high activity across a broad pH range (7.0-9.0) and in the presence of mild denaturants (e.g., low-concentration urea), enabling efficient digestion of complex proteomes - including partially refractory membrane proteins or cysteine-rich proteins.
✅ Convenient Ready-to-Use Formulation
The MS Grade Trypsin Protease is supplied as lyophilized powder or pre-diluted liquid in MS-compatible buffers free of ionization-interfering salts. Upon reconstitution, it can be used directly without additional cleanup steps.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
