GTPase Enrichment Kits with GTP Probes

MtoZ Biolabs GTPase Enrichment Kits with GTP Probes - Precise Capture of GTPase Active States and Dissection of Signaling and Disease Mechanisms

GTPases function as key molecular switches that regulate essential cellular processes such as proliferation, motility, and membrane trafficking. Their active states, defined by binding to either GTP or GDP, determine the activation status of signaling pathways. Built on activated GTP analog probes, the MtoZ Biolabs GTPase enrichment product line selectively and covalently labels GTP-bound GTPases, enabling direct enrichment and dynamic profiling of functionally active GTPase populations. This approach provides a dependable chemical proteomics tool for studying GTPase signaling regulation, identifying novel targetable GTPases, and evaluating inhibitor performance.

Advantages

State-Specific Active GTPase Labeling

In the MtoZ Biolabs GTPase Enrichment Kits with GTP Probes, probes designed to mimic the activated conformation of GTP enable selective and covalent labeling of GTP-bound (active) GTPases, providing a readout of real-time intracellular GTPase activity levels rather than total protein abundance.

Coverage of Key Signaling Nodes

Efficient enrichment of major GTPase families, including Ras, Rho, and Rab, supports the generation of comprehensive active GTPase signaling profiles.

Powerful Tool for Drug Development

The enriched active GTPase pool supports evaluation of cell-based inhibitor performance, including assessment of target occupancy for GTPase-directed small molecules, determination of binding affinities, and verification of specificity and off-target effects through competition-based measurements.

Seamless Fit with Downstream Analytical Workflows

Affinity tags such as biotin facilitate purification workflows, yielding highly purified material suitable for Western blot validation or high-sensitivity mass spectrometry analysis and enabling a smooth transition from discovery to verification.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.