EGS (ethylene glycol bis(succinimidyl succinate)) from MtoZ Biolabs is an amine-reactive homobifunctional crosslinker featuring NHS ester groups at both ends of a 12-atom spacer arm, designed for stable fixation and controlled analysis of protein-protein interactions in biochemical, structural biology, and proteomics research.
Product Overview
In protein interaction and structural studies, spacer length, membrane permeability, and crosslink stability are key factors that influence interaction capture and data interpretation. Among amine-reactive crosslinkers, several inherent considerations commonly affect experimental outcomes:
1. Spacer Length Limitations
Crosslinkers with short or inflexible spacer arms may fail to capture interactions spanning larger distances or flexible protein regions.
2. Balance Between Stability and Reversibility
Fully irreversible crosslinks can complicate downstream analysis, while insufficient stability may lead to loss of interaction information during processing.
3. Solubility Constraints
Some crosslinkers are not water-soluble and require organic solvents for dissolution, which must be carefully managed to preserve protein integrity.
4. Intracellular Accessibility
Membrane permeability is essential for studies aiming to stabilize protein interactions inside intact cells.
Advantages
The MtoZ Biolabs EGS (ethylene glycol bis(succinimidyl succinate)) offers the following advantages:
✅ Defined 12-Atom Spacer for Long-Range Interaction Capture
The extended spacer arm supports stabilization of protein-protein interactions occurring over longer distances, aiding interaction mapping and structural interpretation.
✅ Amine-Reactive NHS Ester Chemistry
NHS ester groups at both ends react specifically with primary amines, including lysine side chains and protein N-termini, forming stable amide bonds under mildly alkaline conditions.
✅ Membrane-Permeable Crosslinking Capability
EGS readily penetrates cellular membranes, enabling intracellular crosslinking to preserve native protein interaction states in intact cells.
✅ Controlled Reversibility Under Defined Conditions
Crosslinks formed by EGS can be cleaved by hydroxylamine treatment at pH 8.5 under elevated temperature, providing an option for controlled reversal during downstream analysis.
✅ Support for Functional Protein Studies
Proteins crosslinked with EGS can retain a substantial level of enzymatic activity following reversible crosslinking, supporting functional and structural investigations.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
