The MS Grade Proteinase K launched by MtoZ Biolabs is a broad-spectrum serine protease that primarily cleaves peptide bonds on the carboxyl side of aliphatic and aromatic amino acids. This proteinase is widely used to remove nuclease contamination during nucleic acid extraction and to efficiently digest complex protein samples prior to mass spectrometry, enabling high-sensitivity proteomics and deeper protein coverage.
Product Overview
During sample preparation for proteomics and nucleic acid extraction, researchers often encounter the following challenges, and standard proteases may fail to deliver reliable performance:
1. Interference from Complex Matrices and Incomplete Digestion
Many biological samples, such as tissues and cell lysates, contain high levels of detergents (e.g., SDS), denaturants (e.g., urea), or salts. Conventional proteases can lose activity under these harsh chemical conditions, leading to incomplete digestion. Residual intact proteins can significantly interfere with downstream LC-MS/MS signals.
2. Autolysis and Impurity-Driven Background Noise
Lower-purity protease preparations may contain autolysis peptides and/or exogenous contaminants. In highly sensitive LC-MS/MS workflows, these impurities can generate substantial background peaks that mask low-abundance targets, increasing the risk of false positives or loss of meaningful identifications.
3. Batch-to-Batch Variability
Activity fluctuations across batches are common for conventional enzyme reagents, resulting in poor consistency in parallel sample processing and reduced long term reproducibility. This is particularly detrimental to quantitative proteomics, where consistency is critical.
Advantages
✅ Exceptional Reproducibility
Unlike standard industrial-grade products, our MS Grade Proteinase K undergoes multi-dimensional biochemical characterization and strict activity testing to ensure strong batch consistency.
✅ Broad Robustness
This proteinase demonstrates broad tolerance to diverse chemical environments, maintaining optimal catalytic activity across a pH range of 7.5 to 12.0 and a temperature window of 20°C to 60°C.
✅ Long-Term Stability
With advanced lyophilization and/or stabilization formulation approaches, MToZ Biolabs' MS Grade Proteinase K retains activity with minimal loss over a storage period of up to 24 months.
✅ High Purity
Through specialized purification, the product is free of nuclease contamination, with no detectable endonuclease, exonuclease, DNase, or RNase activities.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
