The MS Grade Asp-N Protease provided by MtoZ Biolabs is a zinc-dependent metalloprotease that primarily cleaves peptide bonds at the N-terminus of aspartic acid (Asp) residues and can, under specific conditions, also recognize glutamic acid (Glu) and oxidized cysteine sites. The protease is widely used in LC-MS/MS-based proteomics for peptide mapping and protein sequence analysis.
Product Overview
In practical proteomics sample preparation, conventional digestion workflows often struggle to simultaneously achieve adequate peptide coverage, cleavage specificity, and data complementarity. These limitations are mainly reflected in the following aspects:
1.
Insufficient Sequence Coverage
Peptides generated by conventional digestion show limited distribution, making certain protein regions difficult to analyze effectively.
2. Pronounced Cleavage-Site Bias
Strategies dominated by C-terminal cleavage restrict access to N-terminal sequence information, reducing identification depth.
3. Imbalanced Peptide Complexity
Non-ideal peptide lengths or distributions decrease database search efficiency.
4.
Limited Data Complementarity
A lack of peptides complementary to commonly used digestion methods limits the overall completeness of protein identification.
Advantages
✅ Increased Sequence Coverage
N-terminal cleavage at Asp residues generates peptides complementary to conventional digestion strategies, significantly improving protein sequence coverage.
✅ High Specific Activity
MtoZ Biolabs' MS Grade Asp-N Protease exhibits clear and stable cleavage specificity, enabling efficient and controlled protein digestion and enhancing the reliability of mass spectrometry identification.
✅ Stable
The protease maintains consistent enzymatic activity under common sample processing conditions, supporting experimental consistency across different sample types and batches.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
