MtoZ Biolabs’ Competitive Peptides are high-purity, antigen-specific peptides validated by high-resolution mass spectrometry. They precisely compete for and bind to the antigen-recognition site of primary antibodies in immunological assays. They are widely used for specificity verification and negative control setup in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), supporting high-accuracy qualitative protein analysis and antibody target confirmation studies.
Product Overview
Competitive Peptides, often referred to as blocking peptides, are synthetic peptides or recombinant antigen fragments that are identical to the epitope sequence recognized by a specific antibody. They serve as a gold-standard negative control reagent in immunoassays such as WB and IHC. The core mechanism is molecular decoying. By pre-incubating the antibody with the peptide prior to detection, the peptide preferentially occupies the antibody’s antigen-binding site, competitively blocking the antibody from binding endogenous proteins in the sample. If the original signal is abolished after peptide blocking, the signal is confirmed as a specific reaction rather than a false positive.
These products include synthetic peptides targeting linear epitopes, recombinant antigen fragments addressing more complex structural determinants, and modified peptides designed for post-translational modifications such as phosphorylation. They are essential for minimizing non-specific background and ensuring the rigor and reliability of experimental data.
Advantages
Competitive Peptides from MtoZ Biolabs offer the following key strengths in design and production.
✅ Ultra-High Sequence Identity and Specificity
Our peptides are synthesized precisely according to the immunogen sequence of the antibody, enabling high-affinity binding to the antibody paratope.
✅ High Purity
MtoZ Biolabs’ Competitive Peptides are purified by HPLC and verified by MS, typically achieving a purity greater than 95%.
✅ Broad Assay Compatibility
Products are optimized for multiple immunoassay platforms, including WB, IHC, ICC, and IF.
✅ Flexible Customization
For PTM-specific antibodies such as phospho-antibodies or conformation-sensitive antibodies, we provide customized peptide synthesis and recombinant antigen expression services.
✅ Excellent Stability
Advanced lyophilization and protective formulations help maintain structural integrity and chemical stability during long-term low-temperature storage.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.