The DSS, No-Weigh Format from MtoZ Biolabs is a well-established, high-purity, membrane-permeable amine-reactive crosslinker designed to deliver robust and reproducible performance in protein-protein interaction (PPI) studies and bioconjugation workflows. Engineered for both intracellular and in-solution applications, this product provides a dependable chemical solution for capturing native and transient protein interactions with high specificity and efficiency.
Product Overview
In protein interaction research and bioconjugation preparation, the performance of a chemical crosslinker directly determines experimental reliability and efficiency. Conventional crosslinking approaches often encounter several limitations:
1. Insufficient Crosslinking Efficiency and Specificity
Crosslinkers with low purity or suboptimal reactivity may cause nonspecific modifications or poor crosslinking yields, failing to effectively “lock” target interactions and generating complex mixtures that are difficult to interpret.
2. Limited Intracellular Applicability
Many commonly used crosslinkers are not membrane-permeable, restricting their use in probing native intracellular protein interaction networks.
3. Cumbersome Handling and Weighing Errors
Traditional powder-form crosslinkers require precise weighing, are sensitive to moisture, and are prone to significant errors when handling small quantities, ultimately compromising experimental reproducibility.
4. Restricted Experimental Flexibility
Crosslinkers with a single structural design often cannot meet the diverse hydrophobicity requirements of different experimental systems, such as membrane-associated versus intracellular crosslinking.
Advantages
✅ High Purity and Excellent Reactivity
The DSS, No-Weigh Format from MtoZ Biolabs is supplied as a highly pure crystalline reagent (>90% purity, verified by NMR). Its two N-hydroxysuccinimide (NHS) ester groups efficiently and selectively react with primary amines in proteins-such as lysine side chains and N-terminal amino groups-under mild conditions (pH 7-9), forming stable amide bonds and ensuring high-efficiency, site-specific crosslinking.
✅ Outstanding Membrane Permeability
As a hydrophobic crosslinker, DSS readily penetrates cell membranes, making it well suited for in situ crosslinking of intracellular proteins. It enables stabilization of weak or transient protein interactions prior to cell lysis or immunoprecipitation, providing a more faithful snapshot of protein interaction states in living cells.
✅ Convenient No-Weigh Packaging
MtoZ Biolabs offers DSS, No-Weigh Format in a pre-aliquoted, no-weigh format for single or multiple uses. This design eliminates weighing errors, reduces moisture sensitivity and handling risks, simplifies experimental workflows, and improves consistency and reproducibility across experiments.
✅ Broad Application Flexibility
The DSS, No-Weigh Format is suitable for a wide range of applications, including intracellular protein crosslinking prior to cell lysis or immunoprecipitation, stabilization of weak or transient protein-protein interactions in solution, and one-step covalent conjugation of proteins, antibodies, or other amine-containing molecules.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
