LC-SPDP (succinimidyl 6-[3(2-pyridyldithio)propionamido]hexanoate) from MtoZ Biolabs is a heterobifunctional, cleavable crosslinker featuring a long spacer arm designed for reversible conjugation of primary amines and thiol groups, enabling controlled protein labeling, crosslinking, and bioconjugate assembly for structural and proteomics studies.
Product Overview
In protein and biomolecule conjugation, the spacer length and cleavable properties of the crosslinker determine the efficiency and specificity of conjugation reactions. Among commonly used crosslinkers, several key factors should be considered:
1. Inadequate Spacer Length for Larger Protein Complexes
Crosslinkers with short spacer arms may not effectively bridge interactions in larger or more flexible protein complexes, limiting structural insights.
2. Reactivity Issues in Complex Samples
Non-cleavable or less specific reagents can lead to random or non-specific coupling, resulting in complex mixtures that are difficult to analyze and interpret.
3. Difficulty in Modifying Intracellular Proteins
Many crosslinkers fail to penetrate cell membranes, limiting their use in intracellular protein modification or protein-protein interaction studies within live cells.
4. Challenges in Efficient Cleavage for Disassembly
Non-reducible or improperly cleaving linkers hinder the ability to selectively dissociate conjugated proteins during subsequent analyses.
Advantages
The MtoZ Biolabs LC-SPDP (succinimidyl 6-[3(2-pyridyldithio)propionamido]hexanoate) offers the following advantages:
✅ Long Spacer for Enhanced Conjugation Flexibility
The extended 6-carbon spacer arm bridges larger or more flexible protein complexes, enabling more effective crosslinking and improved structural analysis.
✅ Amine- and Thiol-Selective Conjugation
LC-SPDP's NHS ester reacts selectively with primary amines, while the pyridyldithiol group targets sulfhydryl groups, enabling site-specific conjugation with precise targeting.
✅ Reversible Conjugation via Cleavable Disulfide Linkage
The disulfide bond within the spacer arm allows for selective cleavage under reducing conditions, enabling the reversible assembly and disassembly of protein complexes.
✅ Membrane-Permeable for Intracellular Protein Modification
The pyridyldithiol group provides membrane permeability, allowing for intracellular protein labeling and modification, expanding the utility of LC-SPDP for in vivo studies.
✅ Easy Monitoring and Quantification
The thiol-disulfide exchange releases a detectable byproduct (2-pyridinethione) that can be measured at 343 nm, providing a straightforward method for tracking reaction progress and conjugation efficiency.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
