MtoZ Biolabs’ Recombinant Proteases are a portfolio of high-purity, recombinantly expressed proteolytic enzymes designed for controlled protein digestion or site-specific cleavage. These recombinant proteases help improve consistency in proteomics sample preparation and support LC-MS/MS method development and routine QC.
Product Overview
Recombinant proteases are enzymes produced and purified through genetic engineering in heterologous hosts such as E. coli. They typically include commonly used enzymes such as trypsin, Lys-C, Glu-C, and PNGase F. Their core function is to cleave peptide bonds with defined specificity and convert proteins into peptides. Recombinant proteases are essential reagents for mass spectrometry sample preparation and directly influence peptide recovery and sequence coverage. Compared with animal-derived enzymes, they eliminate the risk of animal-origin contaminants and provide a foundation for accurate bottom-up proteomics data.
Advantages
✅ High Purity
Purity ≥ 95%. Minimizes interference from contaminating enzymes and supports single, well-defined cleavage specificity.
✅ Enhanced Enzymatic Activity
Molecularly engineered for higher specific activity, enabling faster and more complete digestion.
✅ High Stability
Modified to resist autolysis, maintaining performance during extended incubations or under denaturing conditions.
✅ Lot-to-Lot Consistency
Standardized manufacturing ensures minimal batch-to-batch variation.
✅ Animal-Free
Free of animal-derived components, reducing contamination risk and supporting biopharmaceutical compliance requirements.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.