MtoZ Biolabs’ Quantitative Fluorometric Peptide Assay is designed for high-sensitivity quantification of peptides or peptide mixtures. The assay is widely used for peptide normalization prior to mass spectrometry (LC-MS/MS) analysis and for a broad range of quantitative proteomics studies.
Product Overview
In proteomics and peptide analysis, accurate peptide quantification is essential, yet conventional approaches face several challenges:
1. Difficulty in Detecting Low-Concentration Peptides
In proteomics workflows, peptide yields after digestion are often low. Traditional UV A280 measurements can be affected by salts and solvents, leading to inaccurate results.
2. Complex Sample Backgrounds
Peptides are frequently present in complex biological matrices, such as serum and cell lysates, where routine methods may fail to provide precise quantification.
3. Heterogeneous Peptide Mixtures
Peptides derived from different proteins vary widely in molecular weight and amino acid composition, which can lead to inconsistent standard curves and compromised quantification.
4. Normalization Issues
Variations in normalization across experiments can affect the accuracy and reproducibility of peptide quantification results.
Advantages
✅ Sensitive
Detects peptides down to the nanogram (ng) level, greatly conserving valuable samples.
✅ Robust
Delivers highly reproducible quantification even under complex experimental conditions, ensuring accurate peptide loading for downstream MS analysis.
✅ Compatible
Works with a wide range of buffer systems and experimental conditions, accommodating diverse sample types.
✅ Convenient
Easy to use and enables rapid quantification, saving time and improving workflow efficiency.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
