MtoZ Biolabs' Desthiobiotin-ATP Probe is an active-site directed nucleotide analog that covalently tags ATP binding proteins and ATPases and enables desthiobiotin-based enrichment for proteome-wide activity and inhibitor engagement profiling.
Product Overview
Activity oriented ATP binding protein profiling is powerful but difficult to execute cleanly. Key challenges include:
1. State Specific Readouts Are Limited
Antibody or single target assays often report total protein or surrogate phosphorylation and cannot directly distinguish functional ATP site engagement across protein families.
2. No Global ATP Binding Activity Map
In complex lysates, it is difficult to unbiasedly capture multiple ATP binding proteins and their functional binding states at the same time, which limits systematic mapping of dynamic ATP dependent activity networks across conditions.
3. Inhibitor Profiling Is Slow and Narrow
Measuring binding selectivity and off target engagement for small molecules Inhibitor typically requires target by target assays with limited throughput and incomplete coverage.
4. Enrichment and Validation Are Not Seamless
Many workflows do not integrate cleanly from probe labeling to pull down to downstream WB or LC-MS, increasing sample loss and reducing interpretability.
Advantages
✔️Active Site Covalent Tagging
The desthiobiotin ATP probe labels conserved ATP pocket residues after endogenous nucleotides are reduced, providing a direct handle on functional nucleotide binding sites rather than total abundance.
✔️Capture of Binding Competent States
The probe labels and enriches ATP binding proteins that remain nucleotide binding competent, regardless of their momentary catalytic activity, enabling a more complete view of functional states across kinases and other ATPases.
✔️Competition Ready Target Engagement Platform
In lysates or complex mixtures the probe can be used in competition formats to compare inhibitor binding trends across many ATP binding proteins in parallel, supporting selectivity and off target assessment.
✔️Dual Mode Downstream Compatibility
MtoZ Biolabs' Desthiobiotin-ATP Probe integrates with streptavidin based enrichment and supports both SDS-PAGE and Western blot validation as well as LC-MS/MS identification and quantitative profiling.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
