MtoZ Biolabs' Lane Marker Sample Buffer is a pre-formulated sample buffer that optimizes SDS-PAGE and Western Blot workflows by enabling real-time monitoring of electrophoresis and transfer with its unique, transferable dyes.
Product Overview
Traditional sample buffers often face limitations that hinder efficient execution of SDS-PAGE and Western Blot experiments.
1. Lack of Process Monitoring
Common dyes like bromophenol blue in traditional buffers do not offer clear visibility of the electrophoresis front, especially in thick gels or fast electrophoresis runs, making it difficult to monitor the progress accurately.
2. Inability to Assess Transfer Efficiency
Standard dyes cannot be transferred onto membranes, leaving the transfer process unmonitored until antibody incubation, preventing early-stage quality control.
3. Limited Loading Flexibility
Traditional 2X buffers limit the volume of sample that can be loaded into each well, often requiring concentration or reducing sample volume for low-concentration samples, thus hindering detection of low-abundance proteins.
4. Dye Compatibility Issues
Some dyes may interfere with subsequent Coomassie Blue or silver staining, or may not be compatible with fluorescence detection systems, limiting their use in multiplex detection workflows.
Advantages
✅ Real-Time Electrophoresis Monitoring
The unique bright pink dye provides a clear and high-contrast tracking of the electrophoresis front, allowing precise control of the electrophoresis run time and efficient monitoring of the progress.
✅ Transferable Dye for Membrane Verification
The pink dye not only tracks the electrophoresis front but also transfers to nitrocellulose or PVDF membranes, offering real-time membrane transfer quality control. This ensures proper membrane orientation, uniform transfer efficiency, and accurate lane alignment, simplifying the subsequent Western Blot procedures.
✅ Enhanced Loading Flexibility with 5X Concentration
The 5X concentration formula of MtoZ Biolabs' Lane Marker Sample Buffer allows for greater sample volume in each well, especially beneficial for low-concentration samples, allowing direct loading without pre-concentration and minimizing sample loss or additional preparation steps.
✅ Wide Dye Compatibility
Fully compatible with Coomassie Blue staining, silver staining, and standard Western Blot procedures, ensuring no interference with downstream detection methods (Note: This product is not compatible with fluorescence detection systems, so should not be used for fluorescent Western Blot).
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
