Integrated Digestion-Desalting Products

The Integrated Digestion-Desalting Products from MtoZ Biolabs are engineered for low-input and precious biological samples, providing a fully enclosed and automation-ready workflow that converts proteins into clean LC-MS-ready peptides within a single self-contained process.

Traditional proteomics sample preparation requires multiple discrete steps, including reduction, alkylation, enzymatic digestion, and desalting, each performed in separate vessels and often with manual transfer operations. These fragmented workflows are time-consuming and prone to peptide loss, contamination, or adsorption during sample handling, which severely compromises the identification and quantification of low-abundance proteins. By contrast, Integrated Digestion-Desalting Products seamlessly consolidates denaturation, reduction/alkylation, enzymatic digestion, and peptide desalting into a unified closed workflow. This streamlined approach dramatically reduces manual intervention, improves reproducibility, and enables efficient processing of samples down to the nanogram scale. The system is ideal for deep coverage proteomics in translational research, precision medicine, and pharmaceutical development, particularly when working with scarce clinical or enriched samples.

Advantages

MtoZ Biolabs' Integrated Digestion-Desalting Products streamline proteomic sample preparation by combining denaturation, reduction/alkylation, enzymatic digestion, and peptide desalting into a unified closed workflow optimized for low-input and automated LC-MS applications.

1. Fully Enclosed, Single-Pass Workflow Minimizes Sample Loss

All pre-processing steps are executed sequentially within a pre-packed or programmable integrated device without intermediate transfers or washing steps. This design significantly reduces adsorption, residual carryover, and contamination risks, enabling efficient recovery from low starting materials.

2. Flexible Multi-Enzyme Options for Customized Digestion Strategies

Multiple high-purity MS-grade proteases, including trypsin, Lys-C protease, and Asp-N protease, allow users to select single-enzyme or combination strategies tailored to protein characteristics, PTM profiling, or quantitative labeling studies.

3. Exceptional Performance with Low-Input Samples

Workflow and sorbent materials are optimized for micro-scale inputs derived from immunoprecipitation (IP), laser microdissection, and other low-abundance sample types, ensuring sufficient peptide yield and purity for high-sensitivity and deep-coverage LC-MS analysis.

4. High-Throughput and Automation Compatible

Available in 96-well plate or micro-chip-based formats, the platform integrates seamlessly with mainstream automated liquid-handling workstations, enabling fully automated processing from sample distribution to LC-MS injection. This greatly improves throughput, parallelism, and data reproducibility.

5. Easy Operation with Standardized Procedures

Pre-loaded reagents or modular components minimize setup complexity. Users simply load samples and follow standardized protocols to obtain LC-MS-ready peptides within hours, reducing operational barriers and manual variability.

Technical Support

1. Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

2. Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

3. FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.