MtoZ Biolabs' Quantitative Colorimetric Peptide Assay is a rapid 15-minute colorimetric quantification solution specifically designed for complex peptide digestion mixtures, offering a sensitive and stable detection workflow.
Product Overview
Accurate quantification of complex tryptic peptide mixtures prior to LC-MS analysis remains a major bottleneck in proteomics sample preparation. Conventional protein quantification assays (such as the standard BCA assay) often exhibit insufficient sensitivity or strong composition-dependent bias toward specific peptide sequences, ultimately compromising quantitative accuracy and reproducibility. The primary challenges include:
1. Limited Sensitivity
Traditional assays display relatively high lower limits of detection for peptides, making it difficult to accurately quantify low-abundance samples.
2. Amino Acid Composition Interference
Synthetic peptides or peptide mixtures containing atypical amino acid compositions can significantly affect chromogenic reactions, resulting in systematic quantification errors.
3. Mismatched Standards
Using intact proteins as calibration standards fails to reflect the chemical behavior of enzymatically digested peptides, leading to non-representative standard curves and inaccurate quantification.
Advantages
✅ High-Sensitivity Detection Optimized for Peptides
The Quantitative Colorimetric Peptide Assay from MtoZ Biolabs leverages an enhanced BCA-based chemistry combined with a proprietary chelating formulation to achieve significantly stronger absorbance signals, typically 3- to 4-fold higher than conventional Micro-BCA peptide assay kits. The method supports a minimum detection limit of 25 μg/mL, and requires only 20 μL of sample to deliver precise quantification within 15 minutes.
✅ Excellent Compatibility with Complex Digest Mixtures
The detection mechanism is specifically designed to respond to the amide backbone of peptides, enabling uniform and reliable performance with complex enzymatic digests such as tryptic peptide mixtures. The kit is also compatible with commonly labeled peptide samples, including TMT-tagged peptides, making it an ideal solution for pre-LC-MS loading normalization.
Note: Not recommended for quantifying single synthetic peptides due to composition-dependent signal variation.
✅ Ready-to-Use Standards Ensure Batch-to-Batch Consistency
Each kit includes a highly optimized 1 mg/mL peptide digest calibration standard, prepared from a mass-spectrometry-grade trypsin digest of a reference protein. Digestion efficiency is tightly controlled with inter-batch quality checks to ensure reproducible chemical behavior that accurately reflects real peptide samples. This enables users to generate reliable standard curves and ensures superior reproducibility and quantitative accuracy in LC-MS workflows.
✅ Rapid, Easy, and Workflow-Friendly Operation
The assay employs a simple mix-and-read format in a 96-well microplate, with fast and stable chromogenic response. Results can be read on standard microplate readers at 480 nm, enabling seamless integration into high-throughput sample preparation pipelines and significantly improving pre-LC-MS processing efficiency.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
