MtoZ Biolabs’ Recombinant Cascade Reagent (rCR) (Kinetic-Chromogenic Assay) is a cascade enzymatic reaction system generated through recombinant expression and specifically designed for endotoxin detection in biologics and pharmaceutical products. This high-sensitivity reagent delivers precise results and is critical for ensuring the safety of injectable drugs, medical devices, and biological materials, as well as compliance with regulatory requirements.
Product Overview
The rCR is a recombinant enzyme system that mimics the reaction mechanism of conventional Limulus Amebocyte Lysate reagents. The system contains recombinant Factor C, Factor B, and proclotting enzyme. Unlike conventional reagents, the rCR system does not contain Factor G, thereby effectively avoiding interference from β-D-glucan and Limulus Amebocyte Lysate-reactive material (LAL-RM) during endotoxin testing.
When endotoxin is present in the sample, recombinant Factor C recognizes and activates Factor B, which subsequently activates the proclotting enzyme and converts it into the active clotting enzyme. This enzyme then cleaves a chromogenic peptide substrate, releasing a color-generating signal that can be detected by a microplate reader, thereby enabling quantitative determination of endotoxin concentration in the sample.
Advantages
✅ High Sensitivity
Capable of detecting endotoxin concentrations as low as 0.005 EU/mL.
✅ High Specificity
The system is highly specific for endotoxin and is not affected by cross-reactivity with (1→3)-β-D-glucan.
✅ Broad Applicability
Suitable for a wide range of sample types, including pharmaceuticals, biological fluids, and medical devices.
✅ Validated Performance
Demonstrated reliability and reproducibility help ensure consistent experimental results.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
