Peptide sample preparation serves as a critical bridge between enzymatic digestion and LC-MS/MS analysis in modern proteomics workflows. Efficient and thorough removal of salts, detergents, buffer components, and other low-molecular-weight contaminants is crucial for enhancing ionization efficiency, minimizing background noise, and ensuring high-quality mass spectrometric data with reliable qualitative and quantitative results. To meet diverse application needs, MtoZ Biolabs offers two professional-grade peptide desalting solutions: a high-performance classical peptide desalting system and a highly integrated digestion-plus-desalting platform. Together, these solutions support scenarios ranging from routine batch processing to the preparation of low-abundance or precious samples, enabling high-purity and high-recovery peptide outputs. By clearing common analytical bottlenecks, MtoZ Biolabs’ peptide desalting solutions ensure clean and MS-ready samples for downstream proteomic analysis.
Classical Peptide Desalting System
The Classical Peptide Desalting System from MtoZ Biolabs utilizes high-capacity reversed-phase or mixed-mode media to selectively retain peptides while efficiently removing salts, buffer components, and other small-molecule contaminants, resulting in clean backgrounds for downstream mass spectrometric analysis. Optimized resin properties and elution conditions enable high recovery even for low-abundance or hydrophobic peptides. Standardized operating procedures ensure consistent batch-to-batch performance and increase analytical reliability across routine proteomic workflows.
Integrated Digestion-Desalting System
The Integrated Digestion–Desalting System from MtoZ Biolabs is an innovative all-in-one platform that consolidates protein reduction/alkylation, enzymatic digestion, and peptide desalting into a unified workflow, making it particularly suitable for low-input samples and high-throughput automation. Within a sealed reaction environment, denaturation, reduction, alkylation, digestion, and desalting are executed sequentially, dramatically reducing sample transfer steps and effectively preventing adsorption losses commonly associated with low-abundance samples. Multiple MS-grade protease options are available, including trypsin, Lys-C protease, and Asp-N protease, allowing researchers to choose single-enzyme or multi-enzyme digestion strategies tailored to their experimental design.
Lab Equipment at MtoZ Biolabs
1. Refrigerated Centrifuge
The Refrigerated Centrifuge from MtoZ Biolabs enables low-temperature operation throughout the entire processing workflow, helping prevent protein degradation and preserving the integrity of protein complexes and post-translational modifications. This controlled environment maintains the biological activity and native characteristics of enriched samples, ensuring higher data authenticity for downstream analysis.
2. Vortex Mixer
The Vortex Mixer from MtoZ Biolabs provides efficient yet gentle mixing to improve binding kinetics and uniformity during immunoprecipitation or affinity enrichment procedures. This enhances enrichment reproducibility, increases workflow reliability, and reduces manual labor, making it an ideal accessory for sample preparation in proteomics research.
3. Vacuum Concentrator
The Vacuum Concentrator from MtoZ Biolabs employs a mild, low-temperature evaporation process to prevent peptide degradation and minimize modification loss during concentration. High processing throughput significantly accelerates pre-LC-MS/MS preparation and improves overall sample handling efficiency for proteomic applications.
Advantages
1. High-Purity Desalting Performance
The Classical Peptide Desalting System from MtoZ Biolabs utilizes high-capacity reversed-phase or mixed-mode media to efficiently retain peptides while thoroughly removing salts, detergents, and small-molecule contaminants. This ensures a clean analytical background and improved signal clarity for LC-MS/MS analysis.
2. High Recovery and Excellent Reproducibility
Optimized resin and elution chemistry deliver consistent high recovery even for low-abundance or hydrophobic peptides. Standardized workflows minimize variability and support reliable batch-to-batch reproducibility in proteomics studies.
3. Simple and Rapid Operation
Flexible device formats enable both single-sample and batch processing without the need for specialized instruments. Peptide cleanup can be completed within minutes, significantly increasing sample preparation throughput.
4. Outstanding Mass Spectrometry Compatibility
Eluted peptides are recovered in MS-compatible organic solvents (e.g., acetonitrile), allowing direct LC-MS injection without drying and reconstitution. This reduces sample loss and shortens total preparation time.
5. Efficient Processing of Low-Input Samples
System performance is optimized for microgram- to nanogram-level inputs, maintaining sufficient peptide recovery for proteomic workflows based on IP, Co-IP, laser microdissection, and other low-starting-material applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.