Enzyme Enrichment Kits and Probes

MtoZ Biolabs Enzyme Enrichment Kits and Probes - An Activity-Based Proteomics Approach for Direct Capture and Analysis of the Active Kinome to Accelerate Targeted Drug Discovery

Kinases serve as central regulators of cellular signal transduction and constitute one of the most important classes of drug targets. Conventional approaches primarily quantify kinase abundance and fail to discriminate between inactive and active states. The kinase activity enrichment product portfolio developed by MtoZ Biolabs leverages innovative ActivX ATP/ADP analog probes to specifically and covalently label the active sites of kinases, thereby enabling the direct enrichment and identification of the active, functional kinome. This technology provides a powerful chemical proteomics platform for comprehensive kinase activity profiling, small-molecule inhibitor target identification and selectivity assessment, as well as mechanistic studies of drug action.

Advantages

Activity-Based Rather than Abundance-Dependent

The probes incorporated in the MtoZ Biolabs Enzyme Enrichment Kits and Probes covalently interact exclusively with kinases that possess intact ATP-binding pockets and preserved catalytic activity, thereby directly reporting kinase activity states and associated conformational features. Compared with conventional antibody-based approaches, this strategy provides results with greater functional relevance.

Flexible Probe Design with Comprehensive Coverage

Broad-spectrum probes enable the simultaneous enrichment and global profiling of multiple kinases, while selective probes support functional studies focused on specific kinase families or subgroups.

Robust Platform for Inhibitor Studies

This platform is suitable for assessing intracellular target engagement of inhibitors, determining half-maximal inhibitory concentrations (IC₅₀), and validating direct molecular targets through competitive binding experiments.

Seamless Compatibility with In-Depth Mass Spectrometry Analysis

The biotinylated probe design allows efficient streptavidin-based enrichment and purification, generating highly purified samples that can be directly subjected to high-sensitivity liquid chromatography-tandem mass spectrometry (LC-MS/MS) for accurate identification and quantitative analysis.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.