MtoZ Biolabs' BSA Digestion Standard, LC-MS Grade is a validated BSA peptide digest standard prepared with MS-grade trypsin and processed through reduction, iodoacetamide alkylation, and C18 desalting. It can be used as a routine QC sample for LC and LC-MS to support proteomics data stability assessment and method development.
Product Overview
In proteomics analysis, data reliability is often constrained by the following uncontrollable factors:
1. System Drift Can Be Hard to Catch Early
Source contamination, unstable spray, column aging, or vacuum changes can reduce signal and shift retention times. Without daily QC, issues may surface only after large runs.
2. Inter-Batch Inconsistency Drives Rework
Small prep or instrument differences between days or operators can skew quantitation and IDs, causing reruns and delays.
3. Method Optimization Becomes Costly
Without a stable standard, system problems can look like method issues during gradient, flow, CE, dynamic exclusion, or window tuning, wasting instrument time and samples.
4. Prep Variability Can Mask Biology
Digestion efficiency, reduction and alkylation conditions, and desalting recovery affect peptide yield and spectral quality, lowering coverage and quantitative stability.
5. Compliance and Delivery Require Traceable QC
Multi-batch projects need reproducible metrics to support data reliability.
Advantages
✅Validated Quality: Optimized to minimize missed cleavages and over-alkylation, with C18 desalting for clean, reproducible LC-MS signals.
✅Excellent Sequence Coverage: Reliable peptide set for tracking IDs, peak shape, response, and retention time.
✅Rigorously Tested: Verified for system suitability and method evaluation to reduce false troubleshooting.
✅Stable: Consistent retention time and signal over long runs, ideal for drift monitoring.
✅No Intact Protein Background: Peptide-focused standard reduces interference from residual intact proteins.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
