The MtoZ Biolabs’ MS Grade Lys-C Protease is specifically designed for site-specific cleavage at the C-terminus of lysine (Lys) residues, facilitating protein sample preparation under highly denaturing conditions and supporting LC-MS/MS–based proteomics analysis.
Product Overview
During proteomics sample preparation, protein structural complexity and processing conditions often introduce multiple technical challenges that can directly affect digestion efficiency and data quality. These challenges mainly include the following aspects:
1. Highly Stable Protein Structures
Highly stable or hydrophobic proteins are difficult to fully unfold, often resulting in incomplete digestion and high missed-cleavage rates.
2. Limitations of Denaturing Systems
Strong denaturants such as high concentrations of urea can inhibit the activity of conventional proteases, compromising digestion consistency.
3. High Peptide Complexity
Non-specific or excessive cleavage increases peptide complexity, reducing database search efficiency.
4. Limited Batch-to-Batch Reproducibility
Digestion conditions are sensitive, and experimental variability can affect quantitative reliability and comparability.
Advantages
✅ High Purity
MtoZ Biolabs’ MS Grade Lys-C Protease is manufactured according to MS Grade purification standards, minimizing contaminating proteins and background interference to ensure accurate and reliable LC-MS/MS data.
✅ Complete Digestion
The Protease provides highly specific cleavage at the C-terminus of lysine (Lys) residues, enabling efficient and controllable protein digestion even under strongly denaturing conditions.
✅ Enhanced Stability
The Lys-C Protease exhibits strong tolerance to common denaturants such as urea, maintaining stable enzymatic activity and improving consistency across samples and batches.
✅ Convenient
The Protease is highly compatible with standard proteomics sample preparation workflows; it can be used alone or as a pre-digestion enzyme prior to trypsin digestion, simplifying experimental design and improving overall efficiency.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
