The MS Grade LysN Protease from MtoZ Biolabs is specifically designed for site-specific cleavage at the N-terminus of lysine (Lys) residues, helping to expand peptide coverage and support LC-MS/MS-based proteomics analysis.
Product Overview
In complex proteomics studies, the choice of digestion strategy directly affects peptide coverage, sequence information acquisition, and the depth and reliability of downstream mass spectrometry identification. The main challenges include:
1. Limited Peptide Coverage
Conventional tryptic digestion shows cleavage bias, resulting in insufficient coverage for certain proteins or regions.
2. Insufficient Sequence Information
Strategies dominated by C-terminal cleavage provide limited access to N-terminal information, affecting protein structure and function studies.
3. Single Digestion Strategy
A single proteolytic approach is often insufficient to meet the requirements of complex samples or specialized research objectives.
4. Limited Data Complementarity
Lack of peptides complementary to tryptic digests restricts identification depth and confidence.
Advantages
✅ Complementary to Tryptic Digests
N-terminal lysine-specific cleavage generates peptide profiles highly complementary to tryptic digestion, enhancing protein identification depth.
✅ High Purity
Manufactured according to MS Grade standards to minimize background interference, making it suitable for high-stringency mass spectrometry analyses.
✅ N-Terminal Lysine Cleavage Specificity
Clear and predictable cleavage sites facilitate database searching and structural information analysis.
✅ Versatile
Applicable to global proteomics, targeted analysis, and structure-related studies; can be used alone or in combination with other proteases.
✅ Stable
Maintains stable enzymatic activity under common sample processing conditions, supporting consistency across different samples and batches.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
