SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) from MtoZ Biolabs is a water-insoluble, heterobifunctional crosslinker with NHS ester and maleimide reactive groups at opposite ends of a cyclohexane spacer, designed for efficient amine-to-sulfhydryl conjugation, protein labeling, and protein-protein interaction studies in biochemical and proteomics research.
Product Overview
In protein and biomolecule conjugation workflows, the choice of crosslinker plays a significant role in determining reaction specificity, efficiency, and downstream compatibility. Several factors should be considered when selecting a crosslinker for amine-sulfhydryl conjugation:
1. Non-Specific Conjugation Risks
Homobifunctional crosslinkers may lead to random conjugation or self-crosslinking, reducing control over conjugation orientation and resulting in heterogeneous products.
2. Irreversible Linkage Constraints
Non-cleavable linkers may restrict flexibility in studying protein-protein interactions after conjugation, making it difficult to separate conjugated proteins or analyze interactions post-crosslinking.
3. Solubility Issues
Crosslinkers that are not water-soluble may require organic solvents, which can complicate their use in biological and aqueous systems.
4. Lack of Monitoring Mechanisms
Crosslinkers without a detectable byproduct make it challenging to assess conjugation efficiency or track the reaction progress in real time.
Advantages
The MtoZ Biolabs SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) provides the following advantages:
✅ Efficient Amine-Sulfhydryl Conjugation
The NHS ester group reacts efficiently with primary amines, such as lysine side chains and protein N-termini, while the maleimide group selectively targets sulfhydryl groups, enabling precise site-specific conjugation.
✅ Cyclohexane Spacer for Enhanced Stability
The cyclohexane-based spacer arm enhances the stability of the maleimide group, improving the crosslinker's performance in protein-protein interaction studies and conjugate formation.
✅ Compatibility With Physiological Conditions
SMCC is readily soluble in organic solvents such as DMF or DMSO, facilitating its use in complex biological systems without the need for harsh solvents.
✅ Stable Maleimide Group for Long-Lasting Conjugates
The maleimide group remains stable for extended periods, ensuring reliable conjugation and minimizing the need for frequent re-preparation during experiments.
✅ High Purity and Consistency
SMCC is produced to high purity standards, and each batch undergoes rigorous quality control to ensure reproducible performance across a range of applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
