Protein digestion represents a critical step in converting intact proteins into peptides and constitutes an essential prerequisite for tandem mass spectrometry (LC-MS/MS)–based protein identification, quantitative analysis, and post-translational modification (PTM) profiling. The efficiency, cleavage specificity, and preservation of sequence information during digestion directly influence the depth of proteome coverage and the accuracy of mass spectrometric measurements. MtoZ Biolabs provides a complete portfolio covering high-purity MS-grade proteases, standardized digestion kits, and comprehensive pretreatment reagents to address challenges commonly encountered in traditional workflows, including insufficient enzymatic activity, non-specific cleavage, sample loss, and interference from complex modifications such as glycosylation. These solutions support the preparation of high-quality and high-recovery peptide samples, thereby enabling in-depth proteomics investigations.
Products
MS-grade Protein Digestion Enzymes
MtoZ Biolabs supplies sequencing-grade, ultra-high purity proteases including trypsin and Lys-C with extremely low autolysis and minimal non-specific cleavage activity, reducing background interference and enabling reliable mass spectrometry-based protein identification.
Protein Digestion Kits
These integrated ready-to-use kits contain optimized buffer systems, high-purity enzymes, and peptide cleanup modules, enabling efficient and standardized digestion and peptide recovery from solutions or gel bands. The workflow simplifies experimental operations and enhances data reproducibility.
Pretreatment Reagents for Digestion
MtoZ Biolabs offers high-purity and mass-spectrometry-compatible pretreatment reagents such as dithiothreitol (DTT) for reducing disulfide bonds and iodoacetamide (IAA) for alkylating cysteine residues. These reagents promote complete denaturation, unfolding, and stabilization of proteins, creating optimal conditions for efficient proteolysis.
Deglycosylation Kits Prior to Digestion
Specifically designed for complex samples such as glycoproteins, these enzymatic de-modification kits (e.g., PNGase F) remove glycan structures prior to proteolysis, thereby reducing interference in enzymatic digestion and LC-MS/MS detection and substantially improving the identification of glycosylation sites and core peptide sequences.
Lab Equipment at MtoZ Biolabs
Refrigerated Centrifuge
Continuous low-temperature centrifugation effectively prevents protein degradation, preserves the integrity of protein complexes and post-translational modifications, and ensures that enriched samples retain their biological activity and physiological authenticity.
Vortex Mixer
Provides efficient yet gentle mixing to enhance the binding efficiency and consistency of immunoprecipitation or affinity-based enrichment workflows, while remaining operationally simple and time-efficient.
Vacuum Concentrator
The gentle low-temperature concentration process minimizes peptide degradation and modification loss. Its high-throughput handling capacity substantially improves the overall efficiency of mass spectrometry sample pretreatment.
pH Meter and Analytical Balance
Used for accurate preparation of pretreatment and digestion buffers to ensure optimal reaction conditions.
Advantages
Excellent Enzymatic Performance
MS-grade proteases exhibit high specificity and catalytic activity without generating autolysis fragments, enabling efficient and accurate peptide production.
High Mass Spectrometry Compatibility
All reagents and buffer components are rigorously selected to avoid introducing ion suppressors or background contaminants, thereby ensuring low-interference signals in downstream LC-MS/MS analysis.
Simplified Standardized Operation
The kit format provides an optimized “sample-in–peptide-out” workflow that substantially reduces method development time and complexity, while supporting high intra-laboratory and inter-batch reproducibility.
Comprehensive Solution
Covers the entire workflow from complex sample pretreatment (including de-modification and reduction-alkylation) through enzymatic digestion and peptide recovery, supporting research objectives ranging from routine protein identification to in-depth modification-focused proteomics.
Technical Support
Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.