MtoZ Biolabs’ Trifluoroacetic Acid (TFA), LC-MS Grade is designed to improve peptide and protein peak shape and separation performance in reversed-phase liquid chromatography. This product is widely used for peptide desalting, protein/peptide RP-HPLC separations, and mobile-phase acidification and ion-pairing system setup at the front end of LC-MS workflows.
Product Overview
In reversed-phase HPLC (RP-HPLC), the separation of peptides and proteins involves multiple physicochemical challenges, and conventional reagents often fail to meet the requirements for high-resolution separations:
1. Peak Tailing and Poor Peak Shape
Peptides/proteins carry strong charge and can undergo secondary interactions with residual silanol groups or metal sites, leading to tailing and peak broadening.
2. Broad Hydrophobicity Range and Severe Co-Elution
In complex samples, peptides vary widely in hydrophobicity, often resulting in insufficient retention or overlapping components, which compromises resolution and quantitative consistency.
3. Difficult Elution of Large or Basic Species
Proteins, peptides, and strongly basic peptides may be overly retained or incompletely eluted in reversed-phase systems, causing carryover and reduced recovery.
4. Low-Input Samples Are More Sensitive to Separation Stability
Low-abundance peptides generate weak signals, and any deterioration in peak shape or increase in baseline noise can amplify quantitative error and reduce identification rates.
Advantages
✅ High Purity
Ultra-high purity reduces background interference and lot-to-lot variation, supporting highly consistent separation performance.
✅ Exceptional Clarity
Processed with stringent filtration and impurity removal to ensure a particle-free solution.
✅ Minimal Baseline Noise (Extremely Low Background Interference)
Optimized for LC-MS, featuring very low UV absorbance and minimal mass spectrometric background noise.
✅ Strict Batch Consistency
Each lot undergoes rigorous quality control testing.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
