The High Sensitivity Colloidal Blue Staining Kit developed by MtoZ Biolabs is specifically designed for stable visualization of low-abundance proteins in gels, enabling clear, high-contrast band detection while remaining compatible with multiple downstream analyses.
Product Overview
Colloidal blue staining is widely used for visualization of protein electrophoresis results due to its low background and good reproducibility. However, in high-sensitivity detection and complex sample analysis scenarios, traditional colloidal blue staining methods still face certain limitations.
1. Long Staining Duration
Conventional colloidal blue staining typically requires extended incubation times, resulting in a less streamlined workflow that is not well suited for high-throughput or rapid analysis needs.
2. Limited Detection of Low-Abundance Proteins
In samples with a wide dynamic range of protein abundance, weak signal bands often exhibit insufficient staining intensity, limiting accurate visual assessment of low-abundance proteins.
3. Limited Compatibility with Gel Types
Polyacrylamide gels of different concentrations or from different sources may show variable staining performance, making it difficult to maintain consistency between experiments.
4. High Requirements for Downstream Compatibility
The staining system must ensure high-quality imaging while avoiding interference with downstream steps such as in-gel digestion or mass spectrometry analysis.
Advantages
✅ Streamlined Workflow and Efficient Operation
The optimized staining system eliminates unnecessary steps, shortening overall experimental time while maintaining reliable staining performance.
✅ Improved Staining Sensitivity
Enhanced detection of low-abundance protein bands allows a more complete representation of protein composition in complex samples.
✅ Low Background and High Contrast
MtoZ Biolabs' high sensitivity colloidal blue staining kit effectively reduces nonspecific background, delivering clear and stable imaging results.
✅ Downstream Analysis Friendly
Stained protein bands can be directly used for in-gel digestion, mass spectrometry identification, and related analyses without introducing additional chemical modifications.
✅ Broad Gel Compatibility
Compatible with gels of different concentrations and formats, providing stable staining performance and supporting standardized experimental workflows.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
