Residue-Specific Recognition
Recognize a defined modification on a specific protein residue, supporting distinction between the modified form and total target protein.
IP-MS Validated PTM-Specific Antibodies for Site-Specific PTM Detection and Enrichment
PTM-specific antibodies recognize a defined post-translational modification at a specific amino acid residue within a known protein sequence. MtoZ Biolabs provides IP-MS validated antibodies for site-specific phosphorylation, acetylation, methylation, crotonylation, lactylation, ubiquitination, and related modifications, supporting Western blotting, immunoprecipitation, co-immunoprecipitation, ChIP, immunofluorescence, and downstream mass spectrometry workflows.
These antibodies can be used to evaluate the modification state of individual proteins, enrich modified target proteins, and support orthogonal verification of PTM sites identified by LC-MS/MS. MtoZ Biolabs also provides PTM proteomics and targeted mass spectrometry services for projects requiring broader site identification or quantitative verification.
PTM-Specific Antibodies for Site-Level Detection and Enrichment
MtoZ Biolabs offers antibodies directed against defined phosphorylation, acetylation, methylation, acylation, and ubiquitination sites on histone and non-histone proteins, with product formats and supported applications varying by target and modification site. The portfolio includes site-specific histone marks and regulatory phosphorylation sites on kinases, receptors, transcription factors, cell-cycle proteins, metabolic enzymes, and other targets. Available formats include monoclonal, polyclonal, and recombinant monoclonal antibodies.
Protein-Context Specificity
Combine the target protein sequence with modification-site recognition rather than detecting the same PTM class across unrelated proteins.
Application-Matched Options
Product options support applications such as WB, IP, Co-IP, ChIP, IF, ICC, IHC, and related workflows, depending on the antibody.
IP-MS Compatibility
Support enrichment of modified target proteins before LC-MS/MS or targeted mass spectrometry-based verification.
PTM Types Supported for Detection and Enrichment
Phosphorylation
Site-specific antibodies for defined phospho-serine, phospho-threonine, and phospho-tyrosine residues, including AKT pT308, ERK1/2 pT202/pY204, EGFR pY1068, γ-H2AX pS139, and additional regulatory sites.
Methylation
Antibodies for defined mono-, di-, and tri-methylation sites on histone and non-histone proteins, including H3K4, H3K9, H3K27, H3K36, H3K79, and related marks.
Acetylation
Site-specific antibodies for lysine acetylation on histone and non-histone proteins, including H3K9ac, H3K18ac, H3K27ac, H4K5ac, H4K8ac, H4K12ac, and additional sites.
Ubiquitination
Antibodies for defined ubiquitination sites, including histone marks such as H2AK119ub and H2BK120ub, for detection, immunoprecipitation, and enrichment workflows.
Crotonylation & Lactylation
Site-specific antibodies for crotonyllysine and lactyllysine marks, including representative histone sites such as H3K14cr and H3K18la.
Other Lysine Acylations
Antibodies for defined propionylation, butyrylation, hydroxybutyrylation, and other site-specific lysine acylation marks available within the portfolio.
Featured IP-MS Validated PTM-Specific Antibodies
Selected products from the MtoZ Biolabs PTM-specific antibody portfolio. Product availability and supported applications vary by target, modification site, and antibody format.
ERK1/2 PT202/Y204
View product → IP-MS Validated Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody
IP · Co-IP · IP-MS compatible
H3K27AC
View product → IP-MS Validated Acetyl-Histone H3 (Lys27) Recombinant Monoclonal Antibody
IP · Co-IP · IP-MS compatible
Where PTM-Specific Antibodies Are Used
PTM-specific antibodies support residue-level analysis of signaling events, chromatin marks, stress responses, metabolic regulation, and other modification-dependent processes.
Signal Transduction & Kinase Activity
Monitor activation-associated phosphorylation sites on receptors, kinases, and downstream effectors, including AKT T308, ERK1/2 T202/Y204, EGFR Y1068, STAT3 S727, and FAK Y397.
Epigenetics & Chromatin Regulation
Study defined histone methylation, acetylation, crotonylation, lactylation, and ubiquitination marks involved in chromatin state, transcriptional regulation, and enhancer activity.
DNA Damage Response & Cell Cycle
Examine γ-H2AX S139, RB S807/811, CDK1 T161, MCM2 S139, SMC1 S957, histone H3 S10, and other sites associated with DNA damage, mitosis, and cell-cycle progression.
Cancer Biology & Therapeutic Response
Evaluate modification states of oncogenic and tumor-regulatory proteins such as EGFR, AKT, ERK, p53, MDM2, c-Myc, NF-κB p65, and Raf1 in cancer models and treatment-response studies.
Metabolism & Mitochondrial Regulation
Investigate PTM sites on ACLY, PDHA1, GSK3, DRP1, and histones carrying metabolite-linked acyl modifications such as lactylation and hydroxybutyrylation.
Immune, Stress & Neurodegeneration Signaling
Study phosphorylation sites on TBK1, IRF3, PERK, eIF2α, HSP27, Tau, TDP-43, and related proteins involved in innate immunity, cellular stress, and neurodegenerative processes.
From PTM-Specific Enrichment to Site-Level Mass Spectrometry Analysis
For projects extending beyond antibody-based detection, MtoZ Biolabs provides PTM proteomics and targeted mass spectrometry services for modified peptide enrichment, PTM site identification, and site-specific quantitative verification.
Frequently Asked Questions
Key considerations for PTM-specific antibody selection, experimental controls, enrichment, and downstream mass spectrometry workflows.
Q1How should I select a PTM-specific antibody?
Q2How do PTM-specific antibodies differ from total protein and pan PTM antibodies?
Q3Can the same PTM-specific antibody be used for both Western blotting and immunoprecipitation?
Q4What controls are recommended for PTM-specific antibody experiments?
Q5How should a PTM-specific signal be interpreted relative to total protein abundance?
Q6Can MtoZ Biolabs support downstream LC-MS/MS or targeted verification?
Find the Right Antibody for Your Research
Selecting the right antibody depends on target specificity, sample species, antibody format, host species, application, validation method, and downstream experimental goals. Use our product filter to quickly identify antibody products that match your experimental requirements.
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