The SDS-PAGE Sample Preparation Kit from MtoZ Biolabs is an all-in-one solution engineered to rapidly remove electrophoresis-interfering substances and concentrate dilute protein samples, enabling clean, reliable, and reproducible SDS-PAGE analysis workflows.
Product Overview
Prior to SDS-PAGE analysis, protein samples are often dissolved or stored in complex buffers containing high concentrations of salts, detergents, denaturants (e.g., urea, guanidine hydrochloride), reducing agents, or other additives. These substances interfere with SDS binding to proteins and frequently lead to precipitation at the loading well, distorted lane morphology, or blurred backgrounds during electrophoresis. Such artifacts reduce separation performance and compromise data interpretation. Traditional cleanup approaches such as dialysis or precipitation are time-consuming, involve multiple steps, and perform poorly when concentrating dilute sample volumes - making them a major bottleneck for achieving optimal gel performance. The SDS-PAGE Sample Preparation Kit from MtoZ Biolabs eliminates these obstacles with an integrated, rapid, and high-efficiency sample conditioning workflow.
Advantages
✅ Efficient Removal of Electrophoresis Interference
MtoZ Biolabs’ SDS-PAGE Sample Preparation Kit utilizes a proprietary PAGE-prep resin that selectively binds proteins in the presence of dimethyl sulfoxide (DMSO), enabling the efficient removal of commonly encountered contaminants including detergents, dyes, reducing agents, carbohydrates, glycerol, guanidine hydrochloride, urea, ammonium sulfate, and extreme pH components. Removing these interference factors at the source prevents gel artifacts and enhances data reliability.
✅ Simultaneous Concentration and Cleanup
Beyond contaminant removal, the kit can concentrate dilute protein samples by up to 6-fold within 20 minutes, making it particularly suitable for low-abundance or low-concentration samples. No ultrafiltration or precipitation steps are required.
✅ Fast and User-Friendly Workflow
The convenient centrifugal cup format facilitates rapid binding, washing, and elution steps. All required components - including PAGE-prep resin, DMSO, centrifugal cups, and compatible elution buffer - are provided for immediate use without auxiliary reagents or protocol customization.
✅ High Recovery and Downstream Compatibility
Protein recovery yields typically range from 75% to 85% in a final elution volume of 50 µL, minimizing sample loss. The elution buffer is fully compatible with BCA protein quantification, enabling accurate normalization before gel loading. Treated samples can be directly mixed with sample loading buffer and loaded directly onto any standard SDS-PAGE system.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
