SPDP (succinimidyl 3-(2-pyridyldithio)propionate) from MtoZ Biolabs is a short-spacer, heterobifunctional, cleavable crosslinker designed for reversible, site-directed amine-thiol conjugation, supporting controlled antibody modification, protein labeling, and bioconjugate assembly in biochemical, immunological, and proteomics-focused research.
Product Overview
In bioconjugation and protein modification workflows, precise control over coupling specificity, reversibility, and product uniformity is essential. For amine-thiol conjugation strategies, several inherent considerations commonly influence experimental outcomes:
1. Directional Control of Conjugation
Homobifunctional reagents may promote random coupling or self-crosslinking, reducing control over conjugation orientation and product homogeneity.
2. Requirement for Reversible Linkage
Non-cleavable linkers permanently join biomolecules, limiting flexibility for downstream separation, validation, or functional analysis.
3. Monitoring of Reaction Progress
Without a detectable reaction byproduct, conjugation efficiency and reaction completion can be difficult to assess.
4. Compatibility With Reducing Conditions
Downstream analytical workflows often involve reducing environments, requiring linkers with well-defined and controllable cleavage behavior.
Advantages
The MtoZ Biolabs SPDP (succinimidyl 3-(2-pyridyldithio)propionate) provides the following advantages:
✅ Improved Control Over Conjugation Specificity
The heterobifunctional design enables sequential amine-thiol coupling, reducing random crosslinking and improving orientation control and conjugate uniformity.
✅ Flexible Workflow Through Reversible Linkage
The reducible disulfide bond allows conjugates to be selectively dissociated when required, supporting downstream validation, component analysis, or functional assessment.
✅ Built-In Reaction Monitoring Capability
Release of a chromogenic byproduct during thiol-disulfide exchange enables straightforward spectrophotometric tracking of conjugation efficiency, improving experimental control.
✅ Expanded Application Scope Requiring Intracellular Access
Membrane permeability supports applications where intracellular protein modification or labeling is required, extending usability beyond surface-restricted workflows.
✅ Reliable Performance and Batch-to-Batch Consistency
High-purity production and rigorous batch verification ensure reproducible conjugation efficiency and consistent experimental outcomes.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
