MtoZ Biolabs' Peptide Substrates are high-purity, sequence-defined research-grade peptide reagents, designed as standardized reaction matrices for protease and modification enzyme reactions, enabling enzyme activity and specificity assessment. They are widely used in enzyme kinetics analysis, inhibitor screening, and pathway mechanism studies.
Product Overview
Peptide Substrates are short peptide molecules designed according to the recognition patterns of target enzymes, containing specific recognition sequences, cleavage sites, or modification sites. Upon reacting with the enzyme, they undergo predictable changes such as peptide bond cleavage or phosphorylation, acetylation, methylation, or glycosylation. These changes convert enzyme activity and substrate preference into measurable signals, commonly used in establishing in vitro reaction systems, method development, and batch consistency quality control. Common types include protease peptide substrates, kinase peptide substrates, transferase and other PTM enzyme peptide substrates, as well as fluorescence, colorimetric, FRET reporter substrates, and isotope or tagged peptide substrates, compatible with plate readers and LC-MS platforms.
Peptide Substrates are key tools for "standardizing, quantifying, and comparing" complex enzyme reactions: On one hand, they provide a well-defined structure and controlled background, reducing variability caused by the heterogeneity of natural substrates; on the other hand, they support the determination of kinetic parameters, selectivity comparison, inhibitor screening, and mechanism dissection, while providing reliable positive controls and methodological benchmarks for LC-MS or plate reader-based detection.
Advantages
✅ Clearly Defined Sequences
Predictable sites and reaction products, facilitating mechanism explanation and result traceability.
✅ High Purity with Low Impurity Background
Reduces non-specific signals, enhancing detection sensitivity and accuracy.
✅ Strong Batch-to-Batch Consistency
Supports cross-batch and cross-project data comparability and reproducibility.
✅ Compatibility with Multiple Detection Platforms
Compatible with fluorescence/colorimetric/FRET, HPLC, and LC-MS readout methods.
✅ Customizable and Expandable
Sequences can be designed based on target enzyme preferences, with options for labeling, modification, or isotope tags to meet various experimental needs.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.