DSSO (Disuccinimidyl Sulfoxide) from MtoZ Biolabs is an MS-cleavable, amine-reactive homobifunctional crosslinker designed to enhance mass spectrometry-based detection, identification, and confident mapping of protein-protein interactions.
Product Overview
In crosslinking mass spectrometry (XL-MS) workflows, the ability to confidently identify crosslinked peptides is strongly influenced by crosslinker chemistry. Among commonly used crosslinkers, several inherent limitations are frequently encountered:
1. High Spectral Complexity From Non-Cleavable Linkers
Crosslinkers that remain intact during fragmentation often generate highly complex MS/MS spectra, making it difficult to distinguish crosslinked peptides from linear or dead-end modified species.
2. Limited Discrimination Between Crosslinked and Non-Crosslinked Peptides
Without characteristic fragmentation signatures, confidently assigning true crosslinked peptide pairs in complex samples can be challenging.
3. Reduced Efficiency in Automated Data Analysis
Ambiguous fragmentation patterns may limit compatibility with database-assisted crosslink identification software and increase false-positive rates.
4. Constraints in Complex or In Situ Systems
In studies involving intact cells, organelles, or complex protein mixtures, insufficient crosslinker cleavability can hinder comprehensive interaction mapping.
Advantages
The MtoZ Biolabs DSSO (Disuccinimidyl Sulfoxide) provides the following advantages:
✅ MS-Cleavable Linker for Simplified Identification
DSSO contains a sulfoxide group that undergoes predictable gas-phase cleavage during collision-induced dissociation, generating characteristic fragment ions that facilitate confident identification of crosslinked peptides.
✅ Improved Sensitivity and Reduced False Positives
The diagnostic fragmentation behavior enables clear discrimination between crosslinked and non-crosslinked peptides, supporting more accurate interaction analysis in complex datasets.
✅ Amine-Reactive NHS Ester Chemistry
Homobifunctional NHS ester groups react specifically with primary amines, such as lysine side chains and protein N-termini, ensuring efficient and consistent crosslink formation.
✅ Compatibility With XL-MS Data Analysis Pipelines
DSSO is well-suited for MS2- and MS2/MS3-based workflows and integrates effectively with commonly used crosslink identification software, supporting scalable and automated analysis.
✅ Support for In Situ and Complex Biological Samples
The membrane-semi-permeable nature of DSSO allows crosslinking in intact cells, organelles, or complex protein assemblies, enabling profiling of native protein interaction networks.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
