High-abundance proteins present in complex biological matrices such as serum and plasma often dominate analytical signals, masking low-abundance proteins that are critical for biomarker discovery and mechanistic studies. The High-Abundance Protein Depletion Kits from MtoZ Biolabs are designed to selectively remove dominant proteins while preserving low-abundance components, thereby enhancing proteome depth and improving the sensitivity of downstream proteomic analyses.
By integrating optimized affinity-based depletion chemistry with mass-spectrometry-compatible workflows, these kits enable researchers to obtain cleaner protein extracts that are better suited for quantitative proteomics, targeted protein analysis, and functional studies.
Core Functionality
1. Selective Removal of High-Abundance Proteins
Efficiently depletes major proteins such as albumin and immunoglobulins, reducing dynamic range and improving the detectability of low-abundance species.
2. Preservation of Proteome Integrity
Optimized conditions minimize non-specific loss and maintain protein integrity, ensuring reliable identification and quantification.
3. MS-Compatible Workflow
Designed for seamless integration with LC-MS/MS-based proteomics as well as downstream applications including Co-IP and Western blotting.
Compatible Sample Types
- Serum and plasma
- Other protein-rich biological fluids
- Pre-cleared cell or tissue-derived protein extracts
The kits are suitable for both discovery-driven and targeted proteomic workflows requiring enhanced sensitivity.
Advantages
1. Enhanced Proteome Coverage
Improves detection of low-abundance proteins by effectively reducing interference from highly abundant components.
2. Reproducible and Reliable Performance
Standardized depletion chemistry ensures consistent results across batches and experimental runs.
3. Streamlined Sample Preparation
Simple and robust protocols reduce hands-on time and experimental variability, supporting high-throughput studies.
4. Broad Downstream Compatibility
Depleted samples are fully compatible with mass spectrometry, immunoassays, and other protein analysis platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
- Abundant Plasma Protein Depletion KitThe abundant plasma protein depletion kit from MtoZ Biolabs employs magnetic nanoparticle-based enrichment technology to selectively recognize and remove major high-abundance proteins from plasma samples.
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