The Desthiobiotin-FP Serine Hydrolase Probe from MtoZ Biolabs is a fluorophosphonate-based covalent labeling probe designed to precisely capture and profile the activity landscape of the serine hydrolase superfamily.
Product Overview
The serine hydrolase superfamily encompasses a diverse group of enzymes - including proteases, lipases, and esterases - that regulate a wide array of physiological and pathological processes. However, functional interrogation of this large enzyme class has traditionally been limited by several bottlenecks:
1. Difficulty in Detecting Active States
Conventional biochemical assays cannot directly and specifically resolve the nucleophilic reactivity of catalytic serine residues, making it challenging to distinguish catalytically active enzymes from inactive precursors or zymogens.
2. Lack of Global Activity Profiling
There is no standard assay capable of unbiased and simultaneous analysis of hundreds of serine hydrolase activities within complex samples.
3. Low-Throughput Inhibitor Characterization
Selectivity and potency profiling of candidate inhibitors typically requires target-by-target biochemical assays, with limited scalability and insufficient coverage for off-target liability assessment.
4. Fragmented Downstream Workflows
Activity detection and deep identification workflows (e.g., LC-MS/MS) are often decoupled, preventing functional validation and target discovery from occurring within the same experimental system.
Advantages
✅ Activity-Site-Specific Covalent Labeling
The Desthiobiotin-FP Serine Hydrolase Probe from MtoZ Biolabs contains a reactive fluorophosphonate (FP) warhead that forms an irreversible covalent bond with catalytic serine residues in the active sites of serine hydrolases. This biochemical selectivity ensures that only catalytically competent enzyme molecules are labeled, enabling direct activity profiling rather than abundance-based detection.
✅ Desthiobiotin Affinity Tag for Reversible Enrichment
The incorporated desthiobiotin tag enables mild, reversible affinity enrichment of labeled proteins for downstream analysis. Enriched fractions are compatible with Western blotting and high-performance LC-MS/MS workflows, improving sensitivity and recovery without introducing harsh elution conditions.
✅ Competitive Inhibitor Profiling Platform
The Desthiobiotin-FP Serine Hydrolase Probe supports competitive binding assays in complex biological matrices (e.g., cell lysates, tissue homogenates, purified recombinant enzymes). By co-incubating the probe with candidate small-molecule inhibitors, one can quantitatively evaluate inhibitor binding affinity and selectivity across the serine hydrolase superfamily, making it an ideal tool for high-efficiency inhibitor screening and off-target risk assessment.
✅ Dual Downstream Analytical Compatibility
The Desthiobiotin-FP Serine Hydrolase Probe offers strong compatibility with dual downstream analytical workflows. Enriched desthiobiotin-labeled proteins can be analyzed by SDS-PAGE and detected with target-specific antibodies to validate known serine hydrolase interactions, enabling Western blot-based confirmation within the same experimental system. Alternatively, enriched proteins can be digested and subjected to LC-MS/MS analysis to achieve global, unbiased characterization of the serine hydrolase proteome, facilitating discovery of novel functional enzymes as well as drug off-targets through high-coverage proteomics.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
