MtoZ Biolabs' TAMRA-FP Serine Hydrolase Probe is a fluorescence tagged activity based probe that covalently labels active serine hydrolases to enable rapid in gel profiling and inhibitor competition studies without additional click tagging steps.
Product Overview
Functional profiling of serine hydrolases is often limited by practical experimental constraints, including:
1. Activity Is Hard to Read Out
Abundance based assays cannot reliably distinguish active enzymes from inactive forms or zymogens.
2. Slow Iteration for Inhibitors
Selectivity and engagement are difficult to compare across conditions when each inhibitor requires multiple target specific assays.
3. Complex Samples Raise Background
In lysates or tissue homogenates, nonspecific signals and incomplete labeling can obscure true activity changes.
4. Disconnected Verification Workflows
Activity measurement and follow up validation are often split across workflows, increasing handling and sample loss.
Advantages
✔️Direct Fluorescent Readout
The TAMRA tag enables immediate visualization of labeled enzymes by SDS-PAGE in gel fluorescence, accelerating method development and sample comparisons.
✔️Active Site Covalent Labeling
The FP warhead reacts with the catalytic serine in functional active sites, providing a true activity centered profile.
✔️Competition Ready Profiling
Co incubation with candidate inhibitors supports competition experiments directly in lysates, tissue homogenates, or purified proteins to assess engagement and selectivity.
✔️Robust Workflow Fit
MtoZ Biolabs TAMRA-FP Serine Hydrolase Probe fits seamlessly into standard activity profiling workflows, supporting rapid screening, gel based quantitation, and straightforward follow up confirmation.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
