BS2G-d0 and BS2G-d4 from MtoZ Biolabs are isotopically paired, amine-reactive homobifunctional crosslinkers designed for mass spectrometry-based identification and quantitative comparison of protein-protein interactions.
Product Overview
For crosslinking experiments coupled with mass spectrometry, crosslinker design directly affects interaction detectability, structural interpretability, and quantitative confidence. Among commonly available crosslinkers, several inherent limitations are frequently encountered:
1. Lack of Isotopic Differentiation
Crosslinkers without isotopic labeling do not support direct comparison between experimental conditions, limiting their use in differential or quantitative interaction studies.
2. Limited Confidence in Crosslinked Peptide Assignment
In complex proteomic samples, the absence of predictable mass offsets can make it difficult to distinguish true crosslinked peptides from background signals.
3. Restricted Structural Resolution
Use of a single spacer chemistry may constrain the ability to infer inter- or intra-molecular distances, reducing the structural information obtainable from crosslinking data.
4. Compatibility With MS-Based Analysis
Some crosslinkers introduce fragmentation or chemical features that complicate peptide identification and downstream data interpretation.
Advantages
The MtoZ Biolabs BS2G-d0 and BS2G-d4 offer the following advantages:
✅ Matched Isotopic Pair for Quantitative Analysis
The d0 and d4 variants form a light and heavy matched set, enabling direct comparison of crosslinked peptides across experimental conditions and supporting quantitative interaction analysis by mass spectrometry.
✅ Defined Short Spacer for Distance Constraints
BS2G features a well-defined short spacer length, allowing stabilization of closely associated protein interactions and providing distance information useful for structural interpretation.
✅ Amine-Reactive NHS Ester Chemistry
Homobifunctional NHS ester groups react specifically with primary amines, such as lysine side chains and protein N-termini, supporting consistent and predictable crosslink formation.
✅ MS-Oriented Design for Confident Identification
The defined mass difference between d0 and d4 crosslinked species simplifies peptide assignment and increases confidence in crosslink identification within complex datasets.
✅ Efficient Use of Limited Sample Material
BS2G-d0/d4 crosslinking workflows are compatible with low microgram protein inputs, providing an effective alternative for interaction and structural studies where sample availability is limited.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
