Protein Digestion Kits

MtoZ Biolabs Protein Digestion Kits: Offering Standardized, End-to-End Digestion Workflows That Streamline the Conversion of Proteins Into Peptides, Thereby Supporting High Reproducibility and High Sample Recovery

Protein enzymatic digestion is a critical but labor-intensive step prior to mass spectrometry analysis, involving multiple procedures including reduction, alkylation, proteolytic digestion, peptide recovery, and purification. Independently optimizing and preparing reagents for each stage is time-consuming and often yields inconsistent results due to variations in operational conditions, which imposes a substantial barrier for laboratories with limited experience. The Protein Digestion Kit from MtoZ Biolabs provides deeply optimized, ready-to-use, and all-inclusive solutions tailored to different sample types (e.g., in-solution samples and gel bands) and digestion enzymes (e.g., trypsin, Lys-C). Each kit integrates all essential high-purity reagents with clear operating instructions, enabling the digestion workflow to be standardized and allowing any laboratory to reliably obtain high-quality peptide samples for downstream mass spectrometry-based protein identification and quantitative analysis.

Advantages

In-Gel Trypsin Digestion Kit

MtoZ Biolabs Protein Digestion Kits are designed to support standardized and reproducible proteomic workflows. The in-gel digestion kit is intended for proteins excised from SDS-PAGE gel bands and provides all required reagents for sequential destaining, reduction-alkylation, proteolytic digestion, and peptide extraction. The optimized workflow enables high-yield recovery of peptides generated from in-gel proteins.

In-Solution Trypsin Digestion Kit

Intended for solution-based protein samples (e.g., cell lysates and immunoprecipitation elution fractions), this kit offers an optimized buffering system that enables efficient and reproducible in-solution proteolysis.

Lys-C Protease Digestion Kit

Developed for applications requiring Lys-C-specific cleavage or dual-enzyme strategies in combination with trypsin to enhance digestion performance, this kit delivers a complete and integrated workflow.

Rapid Digestion Kit

Utilizing performance-enhanced reagents and an accelerated digestion protocol, this kit enables completion of the full enzymatic digestion workflow within a few hours, meeting the requirements of rapid-turnaround experimental schedules.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.