MtoZ Biolabs has launched the Succinylated Peptide Enrichment Kit which is specifically designed for succinylated peptides. It overcomes challenges associated with low abundance and background interference, and supports studies on metabolic regulation and post-translational modifications.
Product Overview
Succinylation predominantly occurs on lysine residues and introduces additional negative charges that markedly alter the physicochemical properties of peptides. These features pose specific challenges for the isolation and enrichment of succinylated peptides from complex biological samples during peptide extraction and analysis.
1. Significant Changes in Physicochemical Properties
The introduction of extra negative charges by succinylation alters peptide isoelectric points and hydrophilicity, which can affect binding behavior and elution stability under conventional enrichment conditions.
2. High Similarity to Acidic Peptides
Succinylated peptides share overall physicochemical characteristics with endogenous acidic peptides, making them prone to competitive binding with non-modified acidic peptides and reducing enrichment selectivity.
3. Highly Concentrated Metabolic Background
Succinylation is frequently distributed among metabolic enzymes and mitochondria-associated proteins. The resulting digested peptide mixtures contain densely populated peptides with similar functions and sequences, increasing the difficulty of downstream signal interpretation.
4. High Sensitivity to Enrichment Conditions
The binding and elution of succinylated peptides are highly dependent on buffer systems and operational parameters. Minor fluctuations in conditions can affect enrichment consistency and batch-to-batch comparability.
Advantages
✅ Modification-Specific Enrichment
The kit is designed around the structural features of succinylation, enabling selective capture of target peptides from complex backgrounds.
✅ Reduced Background Interference
Optimized binding and washing workflows effectively minimize co-enrichment of unmodified and other modified peptides, improving the purity of succinylated peptides.
✅ High Enrichment Stability
The enrichment conditions are systematically optimized and tolerant to variations in buffer composition and operation, delivering consistent and reproducible succinylated peptide enrichment across batches.
✅ Compatibility with Multiple Sample Types
The succinylated peptide enrichment kit from MtoZ Biolabs can be stably applied to digested peptide samples from different sources, with controllable operation conditions and good reproducibility.
✅ Broad Downstream Compatibility
Enriched succinylated peptides can be directly used for LC-MS/MS, Western blotting, immuno-enrichment validation, and further modification site characterization across multiple downstream analytical platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
