The Crotonylated Peptide Enrichment Kit developed by MtoZ Biolabs is specifically designed for the selective enrichment of low-abundance crotonylation peptides, which is helpful for the study of chromatin regulation and posttranslational modification mechanism.
Product Overview
Crotonylation predominantly occurs on lysine residues and is characterized by low overall abundance and highly dynamic regulation. It is mainly distributed in nuclear proteins and chromatin-associated proteins. These features pose specific challenges for the enrichment and characterization of target peptides from digested peptide samples:
1. Limited Modification Signals
Crotonylated peptides account for only a small fraction of the total digested peptide pool and are easily masked by high-abundance unmodified peptides, resulting in insufficient sensitivity for direct detection.
2. Similar Modification Types
Their physicochemical properties are similar to those of acetylation, butyrylation, and other acyl modifications, making effective discrimination under conventional enrichment conditions difficult and compromising enrichment specificity.
3. Complex Nuclear Protein Background
Digested peptide mixtures derived from nuclear proteins of cells or tissues are highly complex, and non-target peptides can readily interfere with selective enrichment.
4. High Requirements for Reproducibility Control
Crotonylation levels are strongly influenced by physiological states, and the enrichment process requires highly stable conditions to ensure the comparability of experimental results.
Advantages
✅ Specific Enrichment Design
An enrichment system is constructed based on the structural and chemical characteristics of crotonylation, enhancing the selective capture of target peptides in complex backgrounds.
✅ Reduced Background Interference
Optimized washing and separation conditions effectively minimize non-specific co-enrichment of unmodified and other acylated peptides, improving signal concentration.
✅ Broad Sample Compatibility
The crotonylated peptide enrichment kit from MtoZ Biolabs can be applied to complex digested peptide samples from different sources, with controllable operating conditions and good result reproducibility.
✅ Good Compatibility with Downstream Analyses
Enriched crotonylated peptides can be directly used for LC-MS/MS, Western blotting, immuno-enrichment validation, and further analysis of modification sites across multiple downstream analytical platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
