The Citrullinated Peptide Enrichment Kit launched by MtoZ Biolabs is specifically designed for citrullinated peptides, overcoming identification challenges caused by charge alterations and supporting post-translational modification studies related to inflammation and autoimmunity.
Product Overview
Citrullination is generated through the deimination of arginine residues, accompanied by charge neutralization and changes in physicochemical properties, and is frequently observed in inflammation- and immunity-related samples. These characteristics pose specific challenges for the enrichment of citrullinated peptides from complex digested peptide mixtures:
1. Low-Abundance Signals
Citrullinated peptides represent an extremely small proportion of the total peptide pool and are often masked by high-abundance unmodified peptide signals, resulting in insufficient sensitivity for direct detection.
2. Pronounced Changes in Charge Properties
The conversion of arginine to citrulline leads to the loss of positive charge, affecting peptide binding behavior under conventional enrichment and separation conditions.
3. Complex Sample Background
Inflammation-associated proteins and structurally similar peptides are abundant in cell, tissue, or biofluid samples, allowing non-target signals to interfere with enrichment selectivity.
4. High Sensitivity to Enrichment Conditions
The binding and elution of citrullinated peptides are sensitive to changes in experimental conditions, which can readily lead to inconsistency in enrichment results across different batches.
Advantages
✅ High-Specificity Enrichment
The enrichment system is optimized based on the characteristics of citrullination, enabling efficient capture of low-abundance target peptides and improving enrichment selectivity.
✅ Reduced Background Interference
The citrullinated peptide enrichment kit launched by MtoZ Biolabs effectively minimizes interference from unmodified and other modified peptides, enhancing enrichment purity.
✅ Good Stability and Reproducibility
Optimized enrichment conditions exhibit strong tolerance to experimental variation, supporting comparability and reproducibility of results across different batches.
✅ Broad Downstream Compatibility
Enriched citrullinated peptides can be directly applied to LC-MS/MS, Western blot, and immuno-enrichment validation, ensuring data accuracy and stability across multiple downstream analytical platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
