The Propionylated Peptide Enrichment Kit launched by MtoZ Biolabs is specifically designed for propionylated peptides, overcoming interference from multiple acyl modifications and supporting studies of chromatin regulation and metabolism-related post-translational modifications.
Product Overview
Propionylation primarily occurs on lysine residues and is characterized by low overall abundance, physicochemical properties similar to several short-chain acyl modifications, and frequent distribution among metabolic and chromatin-associated proteins. These features create specific challenges for enrichment from complex digested peptide mixtures:
1. Weak Modification Signals
Propionylated peptides constitute only a small fraction of the total peptide pool and are easily masked by high-abundance unmodified peptides, resulting in insufficient sensitivity for direct detection.
2. Difficulty Distinguishing from Similar Modifications
Propionylation shares structural and physicochemical similarities with acetylation, butyrylation, and other acyl modifications, leading to the co-enrichment of non-target acylated peptides under conventional conditions.
3. High Sample Background Complexity
Digested peptides derived from cells or tissues contain dense populations of peptides with similar functions and sequences, increasing the difficulty of downstream signal interpretation after enrichment.
4. Strong Dependence on Enrichment Conditions
The binding and elution processes are sensitive to buffer composition and operational parameters, which can affect result consistency across different batches.
Advantages
✅ Propionylation-Specific Enrichment Design
The enrichment system is optimized around the structural features of propionylation, enhancing selective capture of target peptides in complex backgrounds.
✅ Effective Suppression of Non-Target Acylation Interference
Optimized washing and separation procedures reduce co-enrichment of other acylated and unmodified peptides, increasing signal focus and clarity.
✅ Good Enrichment Stability
The propionylated peptide enrichment kit from MtoZ Biolabs is systematically optimized to tolerate operational variability, supporting comparability of results across different batches.
✅ Strong Downstream Compatibility
Enriched propionylated peptides can be directly applied to LC-MS/MS, Western blotting, and immuno-enrichment validation, as well as other downstream analytical applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
