The Dihydroxyisobutyrylated Peptide Enrichment Kit launched by MtoZ Biolabs is specifically designed for low-abundance dihydroxyisobutyrylation modifications, enabling selective enrichment and supporting mass spectrometry-based analysis of this post-translational modification.
Product Overview
Dihydroxyisobutyrylated peptides represent a class of post-translational modifications characterized by low abundance, structural similarity, and complex background interference. Their separation and enrichment from complex peptide digests pose specific experimental challenges.
1. Extremely Low Target Peptide Abundance
Dihydroxyisobutyrylated peptides constitute only a small proportion of the total peptide pool and are often masked by high-abundance unmodified peptides, resulting in insufficient sensitivity for direct detection.
2. Difficulty in Modification Discrimination
This modification shares similar physicochemical properties with multiple acylation modifications, making it prone to the co-enrichment of non-target modified peptides under conventional enrichment conditions.
3. Complex Sample Background
Peptide digests derived from cells or tissues contain complex components, and the presence of salts and high-abundance peptides can interfere with enrichment selectivity and stability.
4. High Reproducibility Requirements
The enrichment process is sensitive to binding and elution conditions, making it difficult to maintain consistent results across different batches.
Advantages
✅ Specific Recognition Design
The kit establishes an enrichment system based on the structural characteristics of dihydroxyisobutyrylation modifications, enhancing the selective capture of target peptides.
✅Reduced Background Interference
Optimized washing and separation procedures minimize the co-enrichment of unmodified and other modified peptides, resulting in more concentrated analytical signals.
✅ Strong Sample Compatibility
The dihydroxyisobutyrylated peptide enrichment kit from MtoZ Biolabs can be stably applied to peptide digests derived from different sources, with controllable operating conditions and good reproducibility of results.
✅ Downstream Analysis Compatibility
The enriched peptides can be directly applied to liquid chromatography-tandem mass spectrometry analysis and support subsequent modification site identification and validation.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
