The Chymotrypsin Digestion Kit launched by MtoZ Biolabs is specifically designed for proteins enriched in hydrophobic and aromatic amino acids, enabling site-specific enzymatic cleavage and supporting improved mass spectrometry coverage of complex protein samples.
Product Overview
Protein samples rich in hydrophobic and aromatic amino acids (such as membrane proteins or structurally dense proteins) face limitations in cleavage sites under conventional digestion conditions. The structural stability and accessibility of these sites present unique challenges for proteolysis:
1. Limited Exposure of Cleavage Sites
Aromatic amino acid sites are often shielded by the hydrophobic core or stable structures of the protein, making it difficult to fully expose these sites under standard conditions, affecting enzymatic cleavage efficiency.
2. Difficulty in Digestion
Some proteins have tightly folded structures, making them more resistant to enzymatic digestion, which can lead to incomplete digestion or incomplete cleavage of certain regions.
3. Large Sample Type Variability
Samples from different sources have varying protein concentrations, background impurities, and buffer systems, complicating the consistency of digestion.
4. High Sensitivity to Conditions
Digestion conditions are highly sensitive to operational and environmental variations, with slight fluctuations potentially impacting the digestion results, reducing repeatability.
Advantages
✅ Specific Cleavage
This kit is specifically designed for the aromatic amino acids, enabling accurate identification and cleavage of target peptides, improving digestion efficiency.
✅ Optimized Reaction System
Optimized digestion conditions enhance the accessibility of cleavage sites, reducing incomplete or excessive digestion, improving digestion efficiency, and increasing peptide coverage.
✅ Strong Adaptability
MtoZ Biolabs' chymotrypsin protease digestion kit is suitable for protein samples from various sources, providing stable digestion performance and good repeatability.
✅ Stability and Consistency
Standardized conditions minimize the impact of operational fluctuations on results, ensuring consistency and reproducibility between batches.
✅ Good Downstream Compatibility
The enriched peptides can be directly used in LC-MS/MS, suitable for subsequent identification, quantification, and post-translational modification studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
