MtoZ Biolabs' Lys-N Protease Digestion Kit is specifically designed for N-terminal specificity cleavage of lysine, enabling efficient and precise digestion, suitable for mass spectrometry analysis and post-translational modification research.
Product Overview
Lys-N protease is characterized by N-terminal specificity cleavage of lysine residues, with cleavage sites being significantly influenced by protein structure and residue accessibility. This creates certain challenges during the digestion process for complex protein samples.
1. Lysine Site Accessibility Variation
Some proteins have lysine sites shielded by stable structures, making them difficult to cleave effectively under standard conditions, affecting digestion integrity.
2. Protein Structural Complexity Impacts Digestion Efficiency
Highly folded or stable proteins limit enzymatic cleavage reactions, leading to incomplete digestion.
3. Significant Differences in Sample Types
Variations in protein composition, concentration, and impurity backgrounds across different sources increase the difficulty in controlling uniform digestion conditions.
4. High Consistency Requirements for Digestion
Lysine N-terminal cleavage is sensitive to reaction conditions, and slight fluctuations in operation conditions may cause inconsistent digestion efficiency, affecting inter-batch experimental comparability.
Advantages
✅ Lys-N Specific Cleavage
Designed for N-terminal lysine specificity cleavage, enabling precise identification and cleavage of lysine sites to produce consistent, predictable peptide products.
✅ Improved Digestion Efficiency
The optimized reaction system enhances the accessibility of lysine sites, improving overall digestion uniformity.
✅ Good Sample Compatibility
MtoZ Biolabs' Lys-N protease digestion kit is suitable for various protein samples from cells, tissues, etc., with stable digestion performance.
✅ Stable and Reproducible Results
Standardized reaction conditions effectively reduce the impact of operational fluctuations, supporting result comparability across batches.
✅ Seamless Integration with Downstream Analysis
Generated peptides can be directly used in LC-MS/MS and other mass spectrometry workflows, meeting the needs for subsequent identification and quantification.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
