The In-Gel Trypsin Digestion Kit from MtoZ Biolabs is a ready-to-use kit for efficient in-gel proteolysis and peptide recovery after gel electrophoresis, enabling confident protein analysis by LC-MS/MS.
Product Overview
In-gel digestion is powerful for resolving complex samples, but it is also easy to lose peptides or introduce variability when steps are done manually. Common challenges include:
1. Complex and Time-Consuming Process
Traditional in-gel digestion involves multiple steps that can be labor-intensive and prone to errors, making it difficult to achieve consistent results.
2. Unstable Results and Poor Reproducibility
Variations in digestion conditions or timing can lead to inconsistent peptide recovery, impacting the reliability and reproducibility of the results.
3. Low Sensitivity and Recovery
Incomplete digestion or peptide loss during gel extraction can result in reduced sensitivity, especially for low-abundance proteins.
4. High Contamination Risk
Contamination from reagents, stains, or detergents used during sample preparation can interfere with digestion efficiency and subsequent analyses.
Advantages
✔️Consistent Digestion Performance
Optimized reagents and standardized steps help improve digestion completeness and reduce missed cleavages.
✔️Higher Peptide Recovery
Streamlined handling and efficient extraction improve yield from small gel pieces and low-input bands.
✔️Cleaner MS Ready Peptides
Built-in cleanup oriented steps reduce stain and detergent carryover, improving LC-MS/MS sensitivity and spectral quality.
✔️Reduced Hands-On Complexity
A guided workflow minimizes timing mistakes and variability across users while keeping the process efficient.
✔️Broad Workflow Fit
MtoZ Biolabs' In-Gel Trypsin Digestion Kit works well with common SDS-PAGE staining workflows and supports downstream LC-MS/MS identification and comparison studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
