The Lys-C Protease Digestion Kit launched by MtoZ Biolabs is used for site-specific cleavage at the C-terminus of lysine residues in complex protein samples, enabling controlled proteolysis and supporting sample preparation for mass spectrometry-based proteomics.
Product Overview
Complex protein samples exhibit substantial variability in structural stability, denaturation strategies, and lysine site accessibility, which poses challenges to the controllability and consistency of site-specific protease digestion:
1. Large Variation in Lysine Site Distribution
The number and spatial distribution of lysine residues differ markedly among proteins, which can result in a wide range of peptide lengths after digestion and affect balanced sequence coverage.
2. Protein Structure Limits Site Accessibility
In structurally stable or highly folded proteins, certain lysine residues are poorly exposed and may be difficult to cleave efficiently under conventional digestion conditions.
3. Interference from Complex Sample Matrices
Protein samples derived from cells, tissues, or biofluids often retain salts, denaturants, or detergents, which can adversely affect the stability of proteolytic reactions.
4. Significant Differences in Sample Conditions
Variations in protein concentration, buffer composition, and processing workflows across sample sources make it challenging to establish uniform digestion conditions.
Advantages
✅ Defined Cleavage Specificity
Site-specific cleavage at the C-terminus of lysine residues facilitates the generation of predictable and structurally consistent peptide products.
✅ Compatibility with Denaturing Conditions
The Lys-C protease digestion kit from MtoZ Biolabs maintains stable activity under commonly used denaturing conditions, supporting the processing of structurally complex or highly stable proteins.
✅ Stable and Reproducible Digestion Performance
Standardized reaction conditions help minimize variability introduced by operational differences, supporting comparability of results across different sample batches.
✅ Seamless Integration with Downstream Analysis
The resulting peptides can be directly applied to LC-MS/MS-based protein identification and quantitative analysis, and support subsequent quantitative studies and post-translational modification analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
