The In-Solution Trypsin Digestion Kit from MtoZ Biolabs is specifically designed for mass spectrometry sample preparation, providing a high-efficiency and comprehensive one-stop solution for protein digestion and derivatization.
Product Overview
In LC-MS-based proteomics, complete and uniform digestion is critical for achieving high sequence coverage, reproducible peptide identification, and reliable quantitative performance. However, traditional in-solution digestion workflows remain labor-intensive, slow, and difficult to standardize across sample types, resulting in common analytical challenges such as:
1. Complex and Time-Consuming Workflows
Reduction, alkylation, digestion, and quenching require separate reagents and multiple hands-on steps, increasing the risk of sample loss and processing errors.
2. Uneven Digestion Efficiency
Conventional digestion methods are often sensitive to sample volume and buffer composition, making it difficult to ensure complete digestion across a broad dynamic concentration range - especially for low-abundance samples.
3. Ionization Imbalance
Lysine-containing peptides typically exhibit lower ionization efficiency compared to arginine-containing peptides, causing biased signal distribution and reducing depth and consistency of sequence coverage.
4. N-Terminal Side Reactions
Some derivatization strategies can introduce unwanted modifications at peptide N-termini, complicating spectra and interfering with peptide identification.
Advantages
✅ Broad and Efficient Proteolysis
The optimized trypsin used in the In-Solution Trypsin Digestion Kit from MtoZ Biolabs enables efficient and consistent protein digestion across a wide concentration range of 0.025-10 µg (approximately 90 reactions), ensuring high digestion performance even for low-input samples.
✅ Highly Specific Guanidination
The guanidination reaction specifically targets the ε-amino group on lysine side chains while minimally modifying peptide N-terminal α-amines, significantly reducing non-target side reactions and spectral interference.
✅ Fully Integrated Reagent System
The In-Solution Trypsin Digestion Kit provides all ready-to-use reagents required for reduction, alkylation, trypsin digestion, and guanidination - eliminating reagent preparation, shortening hands-on time, and reducing variability across operators and experiments.
✅ Rapid End-to-End Sample Processing
The optimized workflow enables complete sample preparation - from protein to derivatized peptides - within a single day, markedly improving laboratory throughput.
✅ Enhanced LC-MS Signal and Coverage Depth
By converting lysine residues into arginine-like functional groups, the derivatization process improves the ionization efficiency of lysine-terminating peptides, producing more balanced MS signal distributions and enabling deeper and more complete sequence coverage in bottom-up proteomics.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
