NEM (N-Ethylmaleimide) provided by MtoZ Biolabs is a highly reactive thiol-modifying reagent that selectively blocks cysteine thiol groups. This NEM helps suppress thiol-related interference and is widely applied in pre-digestion processing and functional studies in proteomics.
Product Overview
During proteomics sample preparation, the protein digestion step is often constrained by thiol reactivity and structural stability, mainly reflected in the following aspects:
1. Nonspecific Reactions Caused by Free Thiols
Cysteine thiol groups are highly reactive and prone to oxidation or nonspecific reactions, interfering with the digestion process.
2. Disulfide Bonds Reducing Proteolytic Efficiency
Disulfide bonds stabilize higher-order protein structures and mask protease cleavage sites, resulting in incomplete digestion.
3. Insufficient Digestion Reproducibility
Variations in thiol states amplify sample-to-sample differences, compromising data consistency and quantitative reliability.
4. Complex Background Signals in Functional Studies
Uncontrolled thiol reactions may obscure true protein functions or enzyme activity changes.
Advantages
✅ Rapid Thiol Blocking
Enables fast and irreversible modification of cysteine thiols under mild conditions.
✅ Reduced Thiol Interference
Prevents thiol oxidation and nonspecific reactions, improving control over protein digestion.
✅ Improved Digestion Consistency
Facilitates more uniform digestion outcomes and enhances data reproducibility.
✅ Broad Research Applicability
Widely used in proteomics sample preparation, enzyme activity analysis, and investigations of thiol-related mechanisms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
