The DTT (Dithiothreitol) from MtoZ Biolabs is a high-purity and highly efficient reducing agent for proteins, providing reliable performance for structural studies and sample preparation workflows.
Product Overview
In proteomics, structural biology, and enzymology research, maintaining cysteine residues in a reduced state is crucial to prevent disulfide bond mispairing, oxidative inactivation, and structural perturbation. Proper reduction is essential for preserving protein activity and ensuring successful downstream analyses such as enzymatic digestion, mass spectrometry, and electrophoresis. Insufficient reduction or the use of low-purity reducing reagents can lead to several issues, including:
1. Loss of Protein Activity
Oxidation of free thiols can inactivate enzymes or functional proteins, compromising enzymatic reaction efficiency or functional assays.
2. Challenges in Structural Analysis
Incomplete disulfide bond reduction can disrupt protein denaturation and unfolding, resulting in abnormal migration during SDS-PAGE or reduced proteolytic digestion efficiency, ultimately lowering MS sequence coverage.
3. Increased Background Interference
Residual impurities in reducing reagents, such as nucleases or phosphatases, may degrade co-purified nucleic acids or interfere with phosphoprotein analysis, introducing unwanted background signals.
Advantages
✅ High-Efficiency Thiol Preservation
The DTT (Dithiothreitol) from MtoZ Biolabs rapidly and quantitatively reduces both intra- and intermolecular disulfide bonds, ensuring that free thiol groups remain fully reduced to restore and maintain the biological activity of enzymes and other proteins.
✅ Broadly Applicable Reducing Performance
At a standard working concentration of 0.1 M, MtoZ Biolabs' DTT (Dithiothreitol) efficiently disrupts disulfide linkages and is fully compatible with SDS-PAGE sample preparation workflows, enabling sharp and accurate protein band resolution.
✅ Exceptional Purity and Analytical Compatibility
This product is free of endonucleases, exonucleases, ribonucleases, and phosphatases, preventing interference with nucleic acid co-precipitates or phospho-protein analyses. Its high purity makes it particularly suitable for integrated multi-omics sample preparation.
✅ Flexible Application Scenarios
MtoZ Biolabs' DTT (Dithiothreitol) is suitable for enzyme stabilization, protein denaturation/reduction, and a wide range of thiol-dependent biochemical reactions and sample preparation workflows, making it a reliable reducing reagent for routine laboratory use.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
