MMTS (Methyl Methanethiosulfonate) is a thiol-reactive compound that enables reversible modification of cysteine and other sulfhydryl groups prior to protein digestion. By transiently blocking thiol reactivity and modulating disulfide bond states, it supports studies of protein structure, enzyme activity, and thiol-dependent functions, and is widely applied in protein chemistry and proteomics analyses.
Product Overview
In proteomics sample preparation and functional studies, inadequate control of thiol states prior to digestion often becomes a critical factor affecting experimental controllability and data interpretation. The main challenges include the following:
1. Excessive Thiol Reactivity Leading to Nonspecific Changes
Free cysteine residues readily undergo oxidation or nonspecific reactions, interfering with the digestion process and functional assessment.
2. Dynamic and Complex Changes in Disulfide Bond and Thiol States
Thiol states continuously change during reduction or digestion, increasing the difficulty of structural and functional analysis.
3. Difficulty in Distinguishing Structural Stabilization from Functional Regulation Effects
Irreversible modifications may obscure true enzyme activity or regulatory mechanisms.
4. Lack of Reversible Regulatory Tools in Functional Studies
Conventional alkylation reagents are not well suited for investigating thiol-dependent dynamic processes.
Advantages
✅ Reversible
Converts thiol groups into the -S-S-CH₃ form, which can be restored by reducing agents such as DTT or TCEP.
✅ Well-Suited
Supports dynamic analysis of thiol-dependent enzyme activity and protein regulatory processes.
✅ Mild
Modification can be achieved under gentle conditions, minimizing perturbation to overall protein structure.
✅ Flexible
Provides a flexible tool for investigating pre-digestion structural regulation, reversible modifications, and functional states.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
