MtoZ Biolabs' Rapid PNGase F Deglycosylation Kit (Denaturing Method) provides an efficient and rapid solution for deglycosylating glycoproteins, addressing the challenges of incomplete reactions and long, complicated procedures typical in traditional methods.
Product Overview
Traditional deglycosylation techniques often require extended incubation times and multiple purification steps, which can compromise protein integrity and reduce sample yield. Key challenges include:
1. Incomplete Deglycosylation
Steric hindrance and inefficient reaction conditions can leave partially glycosylated proteins, complicating analysis.
2. Sample Loss
Time-consuming purification steps lead to sample loss, especially with low-concentration or precious samples.
3. Long Process Time
Traditional methods often require overnight reactions or lengthy clean-up steps, leading to extended processing times.
Advantages
✅ Fast and Efficient
MtoZ Biolabs' Rapid PNGase F Deglycosylation Kit (Denaturing Method) speeds up the deglycosylation process, providing results after just a 10-minute incubation at 50°C. This efficiency makes it ideal for high-throughput workflows.
✅ High Recovery Rate
The simplified protocol minimizes sample loss and ensures high recovery, even with low-concentration glycoproteins.
✅ Deep Deglycosylation
Denaturation fully disrupts protein tertiary structure and exposes all N-glycosylation sites, allowing PNGase F to access and completely remove N-glycans.
✅ Wide Application
Processed samples can be used directly for SDS-PAGE, Western blotting, HPLC, or MS, supporting routine glycoprotein molecular weight assessment, glycosylation site identification, and glycan profiling.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
