MtoZ Biolabs' PNGase F Deglycosylation Kit (Magnetic Bead Method) enables enzymatic removal of N-linked glycans by specifically cleaving the bond between the glycoprotein Asn residue and the GlcNAc, releasing intact N-glycan chains for reliable studies of glycoprotein function, structure, and quality control.
Product Overview
N glycan removal is often straightforward in principle but difficult to execute cleanly and reproducibly in real samples. Common challenges include:
1. Incomplete Deglycosylation
Steric hindrance, compact folding, or suboptimal reaction conditions can leave partially deglycosylated species, complicating band interpretation and peptide level identification.
2. Sample Loss During Cleanup
Multiple transfers and buffer exchange steps can reduce recovery, especially for low input samples or dilute protein preparations.
3. Detergent and Buffer Interference
Solubilization reagents and salts that help keep proteins soluble may suppress enzyme performance or carry into downstream workflows, affecting gel quality and MS sensitivity.
4. Time-Consuming and Complex Procedures
Traditional deglycosylation methods often involve time-consuming steps such as gel filtration, or precipitation, which can complicate the process and extend the overall workflow.
Advantages
✅ Complete Deglycosylation
The PNGase F Deglycosylation Kit (Magnetic Bead Method) from MtoZ Biolabs ensures thorough deglycosylation under both native and denaturing conditions, minimizing incomplete reactions and enhancing peptide identification for a wide range of glycoproteins.
✅ High Sample Recovery
Magnetic bead-based cleanup maximizes sample recovery with minimal loss, ideal for low-input or dilute samples.
✅ Minimal Interference
The kit minimizes detergent and buffer interference, preserving protein solubility and ensuring high-quality results for downstream analysis.
✅ Simplified and Fast Procedure
This method utilizes streptavidin magnetic beads to capture biotinylated PNGase F, which can then be easily removed from the reaction system using a magnetic separation device. This eliminates the need for traditional column chromatography, greatly simplifying the workflow and reducing processing time.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
