MtoZ Biolabs' MS-Compatible Magnetic IP Kit (Streptavidin) provides a streptavidin magnetic bead system optimized for low background enrichment of biotinylated proteins and peptides in LC-MS-based workflows.
Product Overview
Immunoprecipitation for mass spectrometry is often limited by the following challenges:
1. Harsh, MS Incompatible Lysis Conditions
Many biotinylation workflows rely on detergents or denaturants such as SDS that are effective for solubilization but incompatible with LC MS, requiring extra cleanup steps and causing sample loss.
2. Extremely Tight Biotin-Streptavidin Binding
The strong interaction between biotin and streptavidin often demands boiling in SDS, high concentrations of biotin, or other harsh conditions for elution, which can contaminate MS runs and distort quantitative results.
3. Nonspecific Binding and Bead-Derived Background
Proteins that adhere nonspecifically to beads or plastics, as well as peptides leaching from the solid phase, increase background signals and complicate identification of true biotinylated targets.
4. Carryover of Salts, Detergents, and Additives
Incomplete removal of buffer components, salts, and residual detergents from wash steps can interfere with chromatography, suppress ionization, and reduce sensitivity in LC-MS analysis.
Advantages
✔️MS Friendly Lysis and Wash Buffers
The MS-Compatible Magnetic IP Kit (Streptavidin) from MtoZ Biolabs provides buffer formulations that balance effective solubilization of biotinylated proteins with LC-MS compatibility, reducing the need for extensive post-IP cleanup.
✔️High Sensitivity for Low Abundance Targets
Efficient streptavidin capture and low-loss handling help enrich low-level biotinylated proteins and peptides, improving detection in complex lysates.
✔️Low Background
Stringent, MS-compatible washes reduce nonspecific binding and residual reagent contamination, lowering background signals and improving signal-to-noise for true targets.
✔️Minimal Antibody Contamination
Antibody biotinylation followed by streptavidin-based enrichment helps minimize antibody carryover into the eluate compared with conventional IP workflows.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
